FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2001858363> ?p ?o ?g. }

• W2001858363 endingPage "1078" @default.
• W2001858363 startingPage "1067" @default.
• W2001858363 abstract "Immunofluorescence microscopy is a valuable tool for analyzing protein expression and localization at a subcellular level thus providing information regarding protein function, interaction partners and its role in cellular processes. When performing sample fixation, parameters such as difference in accessibility of proteins present in various cellular compartments as well as the chemical composition of the protein to be studied, needs to be taken into account. However, in systematic and proteome-wide efforts, a need exists for standard fixation protocol(s) that works well for the majority of all proteins independent of subcellular localization. Here, we report on a study with the goal to find a standardized protocol based on the analysis of 18 human proteins localized in 11 different organelles and subcellular structures. Six fixation protocols were tested based on either dehydration by alcohols (methanol, ethanol or iso-propanol) or cross-linking by paraformaldehyde followed by detergent permeabilization (Triton X-100 or saponin) in three human cell lines. Our results show that cross-linking is essential for proteome-wide localization studies and that cross-linking using paraformaldehyde followed by Triton X-100 permeabilization successfully can be used as a single fixation protocol for systematic studies." @default.
• W2001858363 created "2016-06-24" @default.
• W2001858363 creator A5005605525 @default.
• W2001858363 creator A5016962487 @default.
• W2001858363 creator A5052101351 @default.
• W2001858363 creator A5059443988 @default.
• W2001858363 creator A5060241561 @default.
• W2001858363 date "2010-04-01" @default.
• W2001858363 modified "2023-10-17" @default.
• W2001858363 title "A single fixation protocol for proteome-wide immunofluorescence localization studies" @default.
• W2001858363 cites W1599125820 @default.
• W2001858363 cites W1963679906 @default.
• W2001858363 cites W1964031596 @default.
• W2001858363 cites W1966863200 @default.
• W2001858363 cites W1973663419 @default.
• W2001858363 cites W1975321314 @default.
• W2001858363 cites W1977475333 @default.
• W2001858363 cites W1988894473 @default.
• W2001858363 cites W2000760321 @default.
• W2001858363 cites W2009854160 @default.
• W2001858363 cites W2011229368 @default.
• W2001858363 cites W2012745831 @default.
• W2001858363 cites W2018415759 @default.
• W2001858363 cites W2048811075 @default.
• W2001858363 cites W2055845386 @default.
• W2001858363 cites W2058379237 @default.
• W2001858363 cites W2059093270 @default.
• W2001858363 cites W2067451034 @default.
• W2001858363 cites W2074477982 @default.
• W2001858363 cites W2087433693 @default.
• W2001858363 cites W2091224218 @default.
• W2001858363 cites W2093203659 @default.
• W2001858363 cites W2106845528 @default.
• W2001858363 cites W2113344523 @default.
• W2001858363 cites W2123762916 @default.
• W2001858363 cites W2135565251 @default.
• W2001858363 cites W2138024261 @default.
• W2001858363 cites W2154562350 @default.
• W2001858363 cites W2156201868 @default.
• W2001858363 cites W2167345891 @default.
• W2001858363 cites W2463904825 @default.
• W2001858363 cites W333834515 @default.
• W2001858363 cites W4238811921 @default.
• W2001858363 doi "https://doi.org/10.1016/j.jprot.2009.10.012" @default.
• W2001858363 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19896565" @default.
• W2001858363 hasPublicationYear "2010" @default.
• W2001858363 type Work @default.
• W2001858363 sameAs 2001858363 @default.
• W2001858363 citedByCount "83" @default.
• W2001858363 countsByYear W20018583632012 @default.
• W2001858363 countsByYear W20018583632013 @default.
• W2001858363 countsByYear W20018583632014 @default.
• W2001858363 countsByYear W20018583632015 @default.
• W2001858363 countsByYear W20018583632016 @default.
• W2001858363 countsByYear W20018583632017 @default.
• W2001858363 countsByYear W20018583632018 @default.
• W2001858363 countsByYear W20018583632019 @default.
• W2001858363 countsByYear W20018583632020 @default.
• W2001858363 countsByYear W20018583632021 @default.
• W2001858363 countsByYear W20018583632022 @default.
• W2001858363 countsByYear W20018583632023 @default.
• W2001858363 crossrefType "journal-article" @default.
• W2001858363 hasAuthorship W2001858363A5005605525 @default.
• W2001858363 hasAuthorship W2001858363A5016962487 @default.
• W2001858363 hasAuthorship W2001858363A5052101351 @default.
• W2001858363 hasAuthorship W2001858363A5059443988 @default.
• W2001858363 hasAuthorship W2001858363A5060241561 @default.
• W2001858363 hasConcept C104317684 @default.
• W2001858363 hasConcept C104397665 @default.
• W2001858363 hasConcept C140051345 @default.
• W2001858363 hasConcept C146249460 @default.
• W2001858363 hasConcept C159654299 @default.
• W2001858363 hasConcept C168240541 @default.
• W2001858363 hasConcept C178790620 @default.
• W2001858363 hasConcept C185592680 @default.
• W2001858363 hasConcept C190062978 @default.
• W2001858363 hasConcept C2776879804 @default.
• W2001858363 hasConcept C2778236600 @default.
• W2001858363 hasConcept C2778723968 @default.
• W2001858363 hasConcept C2780848204 @default.
• W2001858363 hasConcept C46111723 @default.
• W2001858363 hasConcept C54355233 @default.
• W2001858363 hasConcept C55493867 @default.
• W2001858363 hasConcept C70721500 @default.
• W2001858363 hasConcept C86803240 @default.
• W2001858363 hasConcept C94795543 @default.
• W2001858363 hasConcept C95444343 @default.
• W2001858363 hasConceptScore W2001858363C104317684 @default.
• W2001858363 hasConceptScore W2001858363C104397665 @default.
• W2001858363 hasConceptScore W2001858363C140051345 @default.
• W2001858363 hasConceptScore W2001858363C146249460 @default.
• W2001858363 hasConceptScore W2001858363C159654299 @default.
• W2001858363 hasConceptScore W2001858363C168240541 @default.
• W2001858363 hasConceptScore W2001858363C178790620 @default.
• W2001858363 hasConceptScore W2001858363C185592680 @default.
• W2001858363 hasConceptScore W2001858363C190062978 @default.
• W2001858363 hasConceptScore W2001858363C2776879804 @default.
• W2001858363 hasConceptScore W2001858363C2778236600 @default.

• next