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• W2001861213 abstract "You have accessJournal of UrologyInfections/Inflammation of the Genitourinary Tract: Kidney & Bladder1 Apr 2012910 MOTILE ESCHERICHIA COLI EVOKES AN INNATE IMMUNE RESPONSE IN BOTH BLADDER AND VAGINAL CELLS Marcelo Lanz, Ased Ali, Beatriz Suarez M-Falero, Claire Townes, Judith Hall, and Rob Pickard Marcelo LanzMarcelo Lanz Newcastle upon Tyne, United Kingdom More articles by this author , Ased AliAsed Ali Newcastle upon Tyne, United Kingdom More articles by this author , Beatriz Suarez M-FaleroBeatriz Suarez M-Falero Newcastle upon Tyne, United Kingdom More articles by this author , Claire TownesClaire Townes Newcastle upon Tyne, United Kingdom More articles by this author , Judith HallJudith Hall Newcastle upon Tyne, United Kingdom More articles by this author , and Rob PickardRob Pickard Newcastle upon Tyne, United Kingdom More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1006AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Innate immunity, mediated through the NF-κB intracellular signalling pathway, plays a key role in protecting against infection. The NF-κB pathway is modulated by cell surface Toll-like-receptor (TLR) activity in response to bacterial components. Urinary tract infection (UTI) is commonly caused by ascending uropathogenic Escherichia coli (UPEC) which initially colonises the vagina before invading the bladder. We investigated the in vitro effect of E. coli on NF-κB signalling in bladder and vagina epithelium. METHODS RT4 urothelial cells and VK2 E6/E7 vaginal cells were transfected with GFP and luciferase NF-κB reporter genes respectively to use as in vitro models to examine the effects of bacterial challenge on intracellular signalling. Cells were cultured and challenged with motile and non-motile UPEC strains plus E. coli surface components including lipopolysaccharide, peptidoglycan and flagellin for 24 hours. Antibodies to TLR2, TLR4 and TLR5 were used for inhibition. RESULTS A significant NF-κB response in RT-4 urothelial cells was evoked by motile E. coli (strain NCTC-10418) and E. coli flagellin. This response peaked at 4-hours (Mean Luciferase luminescence 4.3 ± 0.36 LU vs 1.4 ± 0.16 LU; t-test P<0.001) and was inhibited by TLR5 antibody but not TLR2 or TLR4 antibody. A similar response to E. coli flagellin was detected in vaginal cells by 8-hours (Mean GFP fluorescence 1.3 ± 0.01 FU vs 1.0 ±0.01 FU; t-test P<0.05). Less motile E. coli strains and other surface components evoked weaker responses. CONCLUSIONS Motile E. coli evokes a powerful innate immune response that is mediated by bacterial flagellin activating TLR5. This response was detected in both vaginal and bladder cells in vitro suggesting a specific, early ‘danger' response to motile infection, with vaginal epithelium playing a key role. The success of this defence mechanism may be crucial in protecting against symptomatic UTI. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e371 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Marcelo Lanz Newcastle upon Tyne, United Kingdom More articles by this author Ased Ali Newcastle upon Tyne, United Kingdom More articles by this author Beatriz Suarez M-Falero Newcastle upon Tyne, United Kingdom More articles by this author Claire Townes Newcastle upon Tyne, United Kingdom More articles by this author Judith Hall Newcastle upon Tyne, United Kingdom More articles by this author Rob Pickard Newcastle upon Tyne, United Kingdom More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ..." @default.
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• W2001861213 title "910 MOTILE ESCHERICHIA COLI EVOKES AN INNATE IMMUNE RESPONSE IN BOTH BLADDER AND VAGINAL CELLS" @default.
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