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• W2001892571 endingPage "e101022" @default.
• W2001892571 startingPage "e101022" @default.
• W2001892571 abstract "Cloning of mammals by somatic cell nuclear transfer (SCNT) is still plagued by low efficiency. The epigenetic modifications established during cellular differentiation are a major factor determining this low efficiency as they act as epigenetic barriers restricting reprogramming of somatic nuclei. In this regard, most factors that promote chromatin decondensation, including histone deacetylase inhibitors (HDACis), have been found to increase nuclear reprogramming efficiency, making their use common to improve SCNT rates. Herein we used valproic acid (VPA) in SCNT to test whether the treatment of nuclear donor cells with this HDACi improves pre- and post-implantation development of cloned cattle. We found that the treatment of fibroblasts with VPA increased histone acetylation without affecting DNA methylation. Moreover, the treatment with VPA resulted in increased expression of IGF2R and PPARGC1A, but not of POU5F1. However, when treated cells were used as nuclear donors no difference of histone acetylation was found after oocyte reconstruction compared to the use of untreated cells. Moreover, shortly after artificial activation the histone acetylation levels were decreased in the embryos produced with VPA-treated cells. With respect to developmental rates, the use of treated cells as donors resulted in no difference during pre- and post-implantation development. In total, five clones developed to term; three produced with untreated cells and two with VPA-treated cells. Among the calves from treated group, one stillborn calf was delivered at day 270 of gestation whereas the other one was delivered at term but died shortly after birth. Among the calves from the control group, one died seven days after birth whereas the other two are still alive and healthy. Altogether, these results show that in spite of the alterations in fibroblasts resulting from the treatment with VPA, their use as donor cells in SCNT did not improve pre- and post-implantation development of cloned cattle." @default.
• W2001892571 created "2016-06-24" @default.
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• W2001892571 date "2014-06-24" @default.
• W2001892571 modified "2023-10-01" @default.
• W2001892571 title "Development to Term of Cloned Cattle Derived from Donor Cells Treated with Valproic Acid" @default.
• W2001892571 cites W1533935872 @default.
• W2001892571 cites W1648861327 @default.
• W2001892571 cites W1935595924 @default.
• W2001892571 cites W1963559963 @default.
• W2001892571 cites W1970289162 @default.
• W2001892571 cites W1970864732 @default.
• W2001892571 cites W1978661875 @default.
• W2001892571 cites W1982372193 @default.
• W2001892571 cites W1992525863 @default.
• W2001892571 cites W1995184255 @default.
• W2001892571 cites W1995856161 @default.
• W2001892571 cites W1996339364 @default.
• W2001892571 cites W1999015942 @default.
• W2001892571 cites W1999038855 @default.
• W2001892571 cites W2001510875 @default.
• W2001892571 cites W2003366415 @default.
• W2001892571 cites W2004393761 @default.
• W2001892571 cites W2010629311 @default.
• W2001892571 cites W2010765881 @default.
• W2001892571 cites W2011301081 @default.
• W2001892571 cites W2012206386 @default.
• W2001892571 cites W2014446373 @default.
• W2001892571 cites W2015969875 @default.
• W2001892571 cites W2016696563 @default.
• W2001892571 cites W2020470063 @default.
• W2001892571 cites W2023745519 @default.
• W2001892571 cites W2028808625 @default.
• W2001892571 cites W2029232146 @default.
• W2001892571 cites W2030701471 @default.
• W2001892571 cites W2038333351 @default.
• W2001892571 cites W2043311354 @default.
• W2001892571 cites W2046453156 @default.
• W2001892571 cites W2047015004 @default.
• W2001892571 cites W2057061914 @default.
• W2001892571 cites W2057430589 @default.
• W2001892571 cites W2059950394 @default.
• W2001892571 cites W2061331718 @default.
• W2001892571 cites W2064310618 @default.
• W2001892571 cites W2064865035 @default.
• W2001892571 cites W2066777575 @default.
• W2001892571 cites W2066996579 @default.
• W2001892571 cites W2070313328 @default.
• W2001892571 cites W2071953998 @default.
• W2001892571 cites W2074781740 @default.
• W2001892571 cites W2079894711 @default.
• W2001892571 cites W2087714970 @default.
• W2001892571 cites W2093155802 @default.
• W2001892571 cites W2095491090 @default.
• W2001892571 cites W2097985864 @default.
• W2001892571 cites W2098249941 @default.
• W2001892571 cites W2099249565 @default.
• W2001892571 cites W2103121079 @default.
• W2001892571 cites W2105093380 @default.
• W2001892571 cites W2105916870 @default.
• W2001892571 cites W2107257929 @default.
• W2001892571 cites W2107277218 @default.
• W2001892571 cites W2113673352 @default.
• W2001892571 cites W2114918609 @default.
• W2001892571 cites W2117691193 @default.
• W2001892571 cites W2119453558 @default.
• W2001892571 cites W2122905958 @default.
• W2001892571 cites W2123476161 @default.
• W2001892571 cites W2125444532 @default.
• W2001892571 cites W2128635667 @default.
• W2001892571 cites W2129081447 @default.
• W2001892571 cites W2132066759 @default.
• W2001892571 cites W2149548143 @default.
• W2001892571 cites W2163798466 @default.
• W2001892571 cites W2164980960 @default.
• W2001892571 cites W2166649689 @default.
• W2001892571 cites W2170614891 @default.
• W2001892571 cites W2174249255 @default.
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• W2001892571 doi "https://doi.org/10.1371/journal.pone.0101022" @default.
• W2001892571 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4069182" @default.
• W2001892571 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24959750" @default.
• W2001892571 hasPublicationYear "2014" @default.
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