FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2001936900> ?p ?o ?g. }

• W2001936900 endingPage "21" @default.
• W2001936900 startingPage "9" @default.
• W2001936900 abstract "The functionally distinct epithelial layers of the ciliary body act as a syncitium to produce the aqueous humour. Ultrastructural studies have shown that the pigmented (PE) and non-pigmented (NPE) cell layers of the ciliary epithelium are connected by gap junctions. However the molecular composition of gap junctions both between and within the two cell layers has not been comprehensively studied. To address this issue the authors have performed an extensive molecular screening of connexin (Cx) expression patterns in ciliary epithelium of the rat. Initially, mRNA was extracted from rat ciliary bodies, reverse-transcribed, and subjected to two rounds of PCR using primer sets designed against each of the 14 Cx isoforms known to be expressed in the rat. This initial screening protocol amplified eight candidate Cx isoforms (Cxs 26, 31, 33, 37, 40, 43, 45 and 46). The Cx isoforms identified in this initial screen were then first assigned to the ciliary epithelium itself (Cxs 26, 31, 40 and 43) or structures outside the epithelium (Cxs 37, 40, and 45) using immunohistochemistry performed on ciliary body whole mounts. No convincing evidence for either Cx 33 or 46 labelling was found in the ciliary body. Then the four Cx isoforms localized to the epithelium were further localized to specific membrane domains within the epithelial cell layers by performing high resolution imaging of the antibody labeling patterns obtained in cryosections. This enabled Cx26 and 31 to be specifically localized to spatially different gap junctions between NPE cells. Cx31 labeled gap junctions associated with an extensive network of membrane interdigitations found between NPE cells at their basal surfaces. In contrast Cx26 labeling in NPE cells was restricted to the basolateral membranes of adjacent NPE cells. Cx40 and Cx43 were both localized to the PE-NPE interface where they formed discrete homomeric/homotypic gap junction plaques. No convincing evidence was found for antibody labeling between PE cells. Thus it appears that intercellular communication, both within the NPE layer and between the PE and NPE cell layers, is mediated by gap junction channels that have distinctive permeability properties. In particular the results raise the possibility that the permeability of PE-NPE gap junctions can be modulated by changing the Cx43 : Cx40 expression ratio. Whether such a change in Cx expression ratios occurs and what effect it has on aqueous humour production and composition remains to be determined." @default.
• W2001936900 created "2016-06-24" @default.
• W2001936900 creator A5003905457 @default.
• W2001936900 creator A5040750518 @default.
• W2001936900 creator A5046539019 @default.
• W2001936900 creator A5081308362 @default.
• W2001936900 date "2002-07-01" @default.
• W2001936900 modified "2023-09-25" @default.
• W2001936900 title "Molecular Profiling and Cellular Localization of Connexin Isoforms in the Rat Ciliary Epithelium" @default.
• W2001936900 cites W1523235079 @default.
• W2001936900 cites W1955666947 @default.
• W2001936900 cites W1967949725 @default.
• W2001936900 cites W1968762452 @default.
• W2001936900 cites W1975666468 @default.
• W2001936900 cites W1999978883 @default.
• W2001936900 cites W2033975920 @default.
• W2001936900 cites W2037819325 @default.
• W2001936900 cites W2052750431 @default.
• W2001936900 cites W2058675712 @default.
• W2001936900 cites W2060871168 @default.
• W2001936900 cites W2061101618 @default.
• W2001936900 cites W2064290617 @default.
• W2001936900 cites W2064382301 @default.
• W2001936900 cites W2069035576 @default.
• W2001936900 cites W2069721951 @default.
• W2001936900 cites W2083763781 @default.
• W2001936900 cites W2089550182 @default.
• W2001936900 cites W2144785163 @default.
• W2001936900 cites W2150066361 @default.
• W2001936900 cites W2185890272 @default.
• W2001936900 cites W2284310155 @default.
• W2001936900 cites W2336197566 @default.
• W2001936900 cites W2415231917 @default.
• W2001936900 cites W2416903008 @default.
• W2001936900 cites W4206472604 @default.
• W2001936900 doi "https://doi.org/10.1006/exer.2002.1187" @default.
• W2001936900 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12123633" @default.
• W2001936900 hasPublicationYear "2002" @default.
• W2001936900 type Work @default.
• W2001936900 sameAs 2001936900 @default.
• W2001936900 citedByCount "37" @default.
• W2001936900 countsByYear W20019369002012 @default.
• W2001936900 countsByYear W20019369002014 @default.
• W2001936900 countsByYear W20019369002016 @default.
• W2001936900 countsByYear W20019369002017 @default.
• W2001936900 countsByYear W20019369002018 @default.
• W2001936900 countsByYear W20019369002019 @default.
• W2001936900 countsByYear W20019369002020 @default.
• W2001936900 countsByYear W20019369002021 @default.
• W2001936900 countsByYear W20019369002022 @default.
• W2001936900 crossrefType "journal-article" @default.
• W2001936900 hasAuthorship W2001936900A5003905457 @default.
• W2001936900 hasAuthorship W2001936900A5040750518 @default.
• W2001936900 hasAuthorship W2001936900A5046539019 @default.
• W2001936900 hasAuthorship W2001936900A5081308362 @default.
• W2001936900 hasConcept C100094513 @default.
• W2001936900 hasConcept C104317684 @default.
• W2001936900 hasConcept C105702510 @default.
• W2001936900 hasConcept C142724271 @default.
• W2001936900 hasConcept C153911025 @default.
• W2001936900 hasConcept C158157758 @default.
• W2001936900 hasConcept C169760540 @default.
• W2001936900 hasConcept C2778434524 @default.
• W2001936900 hasConcept C2780228694 @default.
• W2001936900 hasConcept C2910722709 @default.
• W2001936900 hasConcept C529295009 @default.
• W2001936900 hasConcept C53345823 @default.
• W2001936900 hasConcept C54355233 @default.
• W2001936900 hasConcept C55493867 @default.
• W2001936900 hasConcept C71924100 @default.
• W2001936900 hasConcept C79879829 @default.
• W2001936900 hasConcept C86803240 @default.
• W2001936900 hasConcept C95444343 @default.
• W2001936900 hasConceptScore W2001936900C100094513 @default.
• W2001936900 hasConceptScore W2001936900C104317684 @default.
• W2001936900 hasConceptScore W2001936900C105702510 @default.
• W2001936900 hasConceptScore W2001936900C142724271 @default.
• W2001936900 hasConceptScore W2001936900C153911025 @default.
• W2001936900 hasConceptScore W2001936900C158157758 @default.
• W2001936900 hasConceptScore W2001936900C169760540 @default.
• W2001936900 hasConceptScore W2001936900C2778434524 @default.
• W2001936900 hasConceptScore W2001936900C2780228694 @default.
• W2001936900 hasConceptScore W2001936900C2910722709 @default.
• W2001936900 hasConceptScore W2001936900C529295009 @default.
• W2001936900 hasConceptScore W2001936900C53345823 @default.
• W2001936900 hasConceptScore W2001936900C54355233 @default.
• W2001936900 hasConceptScore W2001936900C55493867 @default.
• W2001936900 hasConceptScore W2001936900C71924100 @default.
• W2001936900 hasConceptScore W2001936900C79879829 @default.
• W2001936900 hasConceptScore W2001936900C86803240 @default.
• W2001936900 hasConceptScore W2001936900C95444343 @default.
• W2001936900 hasIssue "1" @default.
• W2001936900 hasLocation W20019369001 @default.
• W2001936900 hasLocation W20019369002 @default.
• W2001936900 hasOpenAccess W2001936900 @default.
• W2001936900 hasPrimaryLocation W20019369001 @default.
• W2001936900 hasRelatedWork W135909292 @default.
• W2001936900 hasRelatedWork W1543060992 @default.

• next