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• W2001984156 abstract "The rhizosphere of Trifolium repens and Lolium perenne was divided into three fractions: the bulk soil; the soil adhering to the roots; and the washed roots (rhizoplane and endorhizosphere). After isolation and purification of DNA from these fractions, 16S rDNA was amplified by PCR and cloned to obtain a collection of 16S rRNA genes representative of the bacterial communities of these three fractions. The cloned 16S rRNA genes were then partially sequenced and analysed by a molecular phylogenetic approach. Our data show a high diversity in the bulk soil, which is dominated by clones related to Gram-positive, Cytophaga–Flexibacter–Bacteroides, Proteobacteria, and Holophaga–Acidobacterium groups. The ubiquity of members of the Holophaga–Acidobacterium group, which is composed of sequences of yet-uncultivated microorganisms, is confirmed. The plant roots have a selective effect towards the gamma Proteobacteria, to the detriment of the Gram-positive and the Holophaga–Acidobacterium bacteria, leading to a dominance of Pseudomonas. This work shows by a culture independent approach that the phylogenetic diversity decreases in the proximity of plant roots." @default.
• W2001984156 created "2016-06-24" @default.
• W2001984156 creator A5035192334 @default.
• W2001984156 creator A5056647574 @default.
• W2001984156 date "1999-10-01" @default.
• W2001984156 modified "2023-10-01" @default.
• W2001984156 title "Phylogenetic diversity of bacterial communities differing in degree of proximity of Lolium perenne and Trifolium repens roots" @default.
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• W2001984156 doi "https://doi.org/10.1016/s0929-1393(99)00028-1" @default.
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