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- W2001999711 abstract "Cryptococcus neoformans is the etiologic agent of cryptococcal meningitis that causes more than half a million deaths worldwide each year. This capsulated basidiomycetous yeast also serves as a model for micropathogenic studies. The ability to make stable mutants, either via ectopic integration or homologous recombination, has been accomplished using biolistic transformation. This technical advance has greatly facilitated the research on the basic biology and pathogenic mechanisms of this pathogen in the past two decades. However, biolistic transformation is costly, and its reproducibility varies widely. Here we found that stable ectopic integration or targeted gene deletion via homologous replacement could be accomplished through electroporative transformation. The stability of the transformants obtained through electroporation and the frequency of homologous replacement is highly dependent on the selective marker. A frequency of homologous recombination among the stable transformants obtained by electroporation is comparable to those obtained by biolistic transformation (∼10%) when dominant drug selection markers are used, which is much higher than what has been previously reported for electroporation when auxotrophic markers were used (0.001% to 0.1%). Furthermore, disruption of the KU80 gene or generation of gene deletion constructs using the split marker strategy, two approaches known to increase homologous replacement among transformants obtained through biolistic transformation, also increase the frequency of homologous replacement among transformants obtained through electroporation. Therefore, electroporation provides a low cost alternative for mutagenesis in Cryptococcus ." @default.
- W2001999711 created "2016-06-24" @default.
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- W2001999711 date "2014-12-24" @default.
- W2001999711 modified "2023-10-17" @default.
- W2001999711 title "Generation of stable mutants and targeted gene deletion strains in Cryptococcus neoformans through electroporation" @default.
- W2001999711 cites W1555979155 @default.
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- W2001999711 cites W1811665544 @default.
- W2001999711 cites W1824218087 @default.
- W2001999711 cites W1872803163 @default.
- W2001999711 cites W1957562398 @default.
- W2001999711 cites W1967620272 @default.
- W2001999711 cites W1982958726 @default.
- W2001999711 cites W1985052951 @default.
- W2001999711 cites W1985910275 @default.
- W2001999711 cites W1989726078 @default.
- W2001999711 cites W2001430852 @default.
- W2001999711 cites W2003092273 @default.
- W2001999711 cites W2005951027 @default.
- W2001999711 cites W2008477629 @default.
- W2001999711 cites W2017782609 @default.
- W2001999711 cites W2018152833 @default.
- W2001999711 cites W2018416742 @default.
- W2001999711 cites W2025115436 @default.
- W2001999711 cites W2025868705 @default.
- W2001999711 cites W2041731996 @default.
- W2001999711 cites W2050225134 @default.
- W2001999711 cites W2051624901 @default.
- W2001999711 cites W2056093977 @default.
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- W2001999711 doi "https://doi.org/10.1093/mmy/myu083" @default.
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