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• W2002002007 abstract "Faeces from infected animals have been suggested as a potential source of contamination and transmission of prion diseases in the environment. This work describes the development of a procedure for the detection of PrP(res) in stools which is based on a detergent-based extraction and immunoprecipitation (IP). The procedure was evaluated by analyzing TSE-spiked sheep and mice faeces, and proved to be specific for PrP(res) with sensitivities of 5-10 microg of infected brain tissue. In order to analyze the shedding of prions, we studied stools from orally inoculated mice over 4-days post-inoculation and also stools from terminally sick scrapie-infected mice. PrP(res) was only detected in stools shortly after the oral ingestion of TSE agents. The procedure described could be a useful tool for studying the excretion of prions and for evaluating potential environmental contamination by prions." @default.
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• W2002002007 date "2008-09-01" @default.
• W2002002007 modified "2023-10-18" @default.
• W2002002007 title "Excretion of BSE and scrapie prions in stools from murine models" @default.
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• W2002002007 doi "https://doi.org/10.1016/j.vetmic.2008.02.014" @default.
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