FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2002024475> ?p ?o ?g. }

• W2002024475 endingPage "438" @default.
• W2002024475 startingPage "431" @default.
• W2002024475 abstract "The 16S rRNA genes of two Chlamydia pneumoniae and two C. psittaci strains of different serovars were sequenced then compared to previously reported Chlamydia 16S rRNA gene sequences. Chlamydia pneumoniae-specific regions were identified and specific primers for nested PCR were synthesized. Nested PCR reactions were performed, in a single tube, by varying the annealing temperature of the amplification cycles. The initial thermal cycles were selected to allow annealing and extension of only the outer primer pair, whilst in later cycles a temperature that allowed inner primer annealing was employed. The inner primers were labelled, one with biotin and the other with fluorescein and consequently the dual labelled amplicon could be immobilized onto antibiotin-coated microtitre plates and detected colorimetrically via an antifluorescein-enzyme conjugate. The assay was found to be sensitive and specific. No cross reactions were observed with C. trachomatis, C. psittaci or other common respiratory pathogens." @default.
• W2002024475 created "2016-06-24" @default.
• W2002024475 creator A5064933791 @default.
• W2002024475 creator A5077438044 @default.
• W2002024475 creator A5080203252 @default.
• W2002024475 creator A5089011216 @default.
• W2002024475 date "1996-04-01" @default.
• W2002024475 modified "2023-09-24" @default.
• W2002024475 title "Development of a simplified polymerase chain reactionenzyme immunoassay for the detection of Chlamydia pneumoniae" @default.
• W2002024475 cites W1482864094 @default.
• W2002024475 cites W1600136521 @default.
• W2002024475 cites W1634406801 @default.
• W2002024475 cites W1801533342 @default.
• W2002024475 cites W1830110554 @default.
• W2002024475 cites W1842078396 @default.
• W2002024475 cites W1906341488 @default.
• W2002024475 cites W1957261952 @default.
• W2002024475 cites W1987834144 @default.
• W2002024475 cites W2012234940 @default.
• W2002024475 cites W2016709286 @default.
• W2002024475 cites W2048211434 @default.
• W2002024475 cites W2054423031 @default.
• W2002024475 cites W2054948523 @default.
• W2002024475 cites W2055951265 @default.
• W2002024475 cites W2077520001 @default.
• W2002024475 cites W2085461799 @default.
• W2002024475 cites W2099909807 @default.
• W2002024475 cites W2108025692 @default.
• W2002024475 cites W2140101891 @default.
• W2002024475 cites W2148369686 @default.
• W2002024475 cites W2155062978 @default.
• W2002024475 cites W2166229810 @default.
• W2002024475 cites W2168591865 @default.
• W2002024475 cites W4247695370 @default.
• W2002024475 doi "https://doi.org/10.1111/j.1365-2672.1996.tb03239.x" @default.
• W2002024475 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8849645" @default.
• W2002024475 hasPublicationYear "1996" @default.
• W2002024475 type Work @default.
• W2002024475 sameAs 2002024475 @default.
• W2002024475 citedByCount "23" @default.
• W2002024475 countsByYear W20020244752013 @default.
• W2002024475 countsByYear W20020244752014 @default.
• W2002024475 countsByYear W20020244752015 @default.
• W2002024475 countsByYear W20020244752021 @default.
• W2002024475 crossrefType "journal-article" @default.
• W2002024475 hasAuthorship W2002024475A5064933791 @default.
• W2002024475 hasAuthorship W2002024475A5077438044 @default.
• W2002024475 hasAuthorship W2002024475A5080203252 @default.
• W2002024475 hasAuthorship W2002024475A5089011216 @default.
• W2002024475 hasConcept C104317684 @default.
• W2002024475 hasConcept C153911025 @default.
• W2002024475 hasConcept C157431215 @default.
• W2002024475 hasConcept C159047783 @default.
• W2002024475 hasConcept C159654299 @default.
• W2002024475 hasConcept C178790620 @default.
• W2002024475 hasConcept C185592680 @default.
• W2002024475 hasConcept C194857396 @default.
• W2002024475 hasConcept C2777391075 @default.
• W2002024475 hasConcept C2777563447 @default.
• W2002024475 hasConcept C2777667758 @default.
• W2002024475 hasConcept C2778419347 @default.
• W2002024475 hasConcept C2778651837 @default.
• W2002024475 hasConcept C2780122209 @default.
• W2002024475 hasConcept C2780420688 @default.
• W2002024475 hasConcept C42062724 @default.
• W2002024475 hasConcept C49105822 @default.
• W2002024475 hasConcept C512185932 @default.
• W2002024475 hasConcept C54355233 @default.
• W2002024475 hasConcept C67905577 @default.
• W2002024475 hasConcept C8185291 @default.
• W2002024475 hasConcept C86803240 @default.
• W2002024475 hasConcept C89423630 @default.
• W2002024475 hasConceptScore W2002024475C104317684 @default.
• W2002024475 hasConceptScore W2002024475C153911025 @default.
• W2002024475 hasConceptScore W2002024475C157431215 @default.
• W2002024475 hasConceptScore W2002024475C159047783 @default.
• W2002024475 hasConceptScore W2002024475C159654299 @default.
• W2002024475 hasConceptScore W2002024475C178790620 @default.
• W2002024475 hasConceptScore W2002024475C185592680 @default.
• W2002024475 hasConceptScore W2002024475C194857396 @default.
• W2002024475 hasConceptScore W2002024475C2777391075 @default.
• W2002024475 hasConceptScore W2002024475C2777563447 @default.
• W2002024475 hasConceptScore W2002024475C2777667758 @default.
• W2002024475 hasConceptScore W2002024475C2778419347 @default.
• W2002024475 hasConceptScore W2002024475C2778651837 @default.
• W2002024475 hasConceptScore W2002024475C2780122209 @default.
• W2002024475 hasConceptScore W2002024475C2780420688 @default.
• W2002024475 hasConceptScore W2002024475C42062724 @default.
• W2002024475 hasConceptScore W2002024475C49105822 @default.
• W2002024475 hasConceptScore W2002024475C512185932 @default.
• W2002024475 hasConceptScore W2002024475C54355233 @default.
• W2002024475 hasConceptScore W2002024475C67905577 @default.
• W2002024475 hasConceptScore W2002024475C8185291 @default.
• W2002024475 hasConceptScore W2002024475C86803240 @default.
• W2002024475 hasConceptScore W2002024475C89423630 @default.
• W2002024475 hasIssue "4" @default.
• W2002024475 hasLocation W20020244751 @default.
• W2002024475 hasLocation W20020244752 @default.

• next