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- W2002024676 abstract "Two techniques for the iodination of methotrexate are described, involving covalent linkage to the drug of 125I-labelled N-succinimidyl-3-(4-hydroxyphenyl)propionate (Bolton and Hunter reagent), or to 125I-labelled histamine. A rapid highly specific radioimmunoassay for methotrexate was developed, employing a specific antiserum covalently linked to magnetisable particles, and 125I-labelled methotrexate as tracer. Incubation times for the assays were 60 and 10 min for the Bolton and Hunter reagent-linked methotrexate and 125I-labelled histamine-linked methotrexate respectively. Separation of bound from free antigen was achieved by a rapid magnetic separation system. Results obtained for serum samples correlated closely with those using an enzymatic (dihydrofolate reductase) competitive protein binding assay for methotrexate. A major advantage of the assay is its potential for processing large numbers of samples rapidly, making it highly suitable for routine clinical use." @default.
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- W2002024676 date "1978-10-01" @default.
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- W2002024676 title "Preparation of 125iodine-labelled methotrexate and its use in a magnetisable particle solid-phase radioimmunoassay" @default.
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- W2002024676 doi "https://doi.org/10.1016/0009-8981(78)90335-2" @default.
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