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- W2002029757 abstract "The blood clotting mechanism of the toad Bufo marinus was investigated in vitro using techniques commonly employed in the study of the prothrombin-thrombin and fibrinogen-fibrin stages of human blood coagulation. Clotting of the toad's blood depends upon the presence of calcium ions. The optimum pH is 7·8 and the optimum temperature 35°–40° C. The clot is jelly-like rather than fibrin-like and is formed by the coagulation of a “fibrinogen”. Clotted blood becomes fluid again in 2–4 hours through the action of a serum lysin. A prothrombin which is converted quantitatively to a thrombin was demonstrated. The prothrombin is converted by a toad blood leucocyte thromboplastin which is as active as some toad tissue extracts. One-stage techniques using intrinsic (i.e. blood) sources of thromboplastin revealed no plasma factors similar to human factor V or to anti-haemophilic globulin. Some of the principles to be followed in the investigation of the blood coagulation mechanism of an animal such as the toad are discussed." @default.
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- W2002029757 date "1961-02-01" @default.
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- W2002029757 title "THE CLOTTING OF THE BLOOD OF AN AMPHIBIAN, BUFO MARINUS LINN" @default.
- W2002029757 doi "https://doi.org/10.1038/icb.1961.6" @default.
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