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- W2002042584 abstract "A psychrotolerant strain, 8H1 T , was isolated from soil samples collected in Isla de los Estados, Ushuaia, Argentina. Cells were Gram-negative, aerobic, straight rods, occurring singly or in pairs, non-spore-forming and motile by means of two polar flagella. The isolate was able to grow in the range 4–35 °C, with optimum growth at 28 °C. The predominant cellular fatty acids were summed feature 3 (C 16 : 1 ω6 c and/or C 16 : 1 ω7 c ), C 16 : 0 and summed feature 8 (C 18 : 1 ω6 c and/or C 18 : 1 ω7 c ). The polar lipid pattern of strain 8H1 T comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. The DNA G+C content was 59.8 mol%. 16S rRNA gene sequence-based phylogeny suggested the affiliation of strain 8H1 T to the ‘ Pseudomonas fluorescens group’, displaying ≥98.5 % sequence similarity to 29 type strains. A multilocus sequence analysis (MLSA) study performed by concatenating 16S rRNA, gyrB , rpoD and rpoB gene sequences showed that isolate 8H1 T could be discriminated from closely related species of the genus Pseudomonas and placed in the ‘ Pseudomonas gessardii subgroup’, including the species with the highest MLSA sequence similarities: Pseudomonas brenneri (96.2 %), P. gessardii (96.1 %), P. proteolytica (96.0 %), P. meridiana (96.0 %) and P. mucidolens (95.4 %). DNA–DNA hybridization analysis between 8H1 T and the type strains of these closely related species revealed relatedness values of 27.0, 8.8, 41.2, 39.7 and 46.1 %, respectively. These results, together with differences in several phenotypic features, support the classification of a novel species, for which the name Pseudomonas yamanorum sp. nov. is proposed. The type strain is 8H1 T ( = DSM 26522 T = CCUG 63249 T = LMG 27247 T )." @default.
- W2002042584 created "2016-06-24" @default.
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- W2002042584 date "2015-02-01" @default.
- W2002042584 modified "2023-10-08" @default.
- W2002042584 title "Pseudomonas yamanorum sp. nov., a psychrotolerant bacterium isolated from a subantarctic environment" @default.
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- W2002042584 doi "https://doi.org/10.1099/ijs.0.065201-0" @default.
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