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• W2002057438 abstract "The demand for molecular analysis of aquatic microbial communities in freshwater has highlighted the need for efficient methods of DNA extraction. The centrifugation method and filtration-membrane method are 2 widely used methods for extracting DNA. The objective of this study was to compare the extraction efficiency of 3 methods, including the centrifugation method, filtration-membrane method, and modified filtration-membrane method, by evaluating the quantity and purity of DNA extracts obtained from water. DNA extraction was analyzed by agarose gel electrophoresis, ultraviolet-spectroscopy, restriction enzyme digestion, and polymerase chain reaction. The results showed that the modified filtration-membrane method was the most efficient for extracting microbial DNA from freshwater with high integrity and purity and is suitable for molecular applications." @default.
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• W2002057438 date "2015-01-01" @default.
• W2002057438 modified "2023-10-16" @default.
• W2002057438 title "Comparison of extraction methods of total microbial DNA from freshwater" @default.
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• W2002057438 doi "https://doi.org/10.4238/2015.january.30.16" @default.
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