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- W2002068942 abstract "Three different extraction methods were compared for the determination of lipids in both oxidised and antioxidant-stabilised brown fish meal. The three methods under investigation were those of Smith-Ambrose-Knobl using chloroform/methanol/water, Bligh and Dyer also using chloroform/methanol/water but monophasic, and Hara and Radin using hexane/isopropanol. The fat content, determined gravimetrically, was significantly lower (P < 0.05) when hexane/isopropanol was used for lipid extraction from both oxidised and antioxidant-stabilised fish meal, than when either of the chloroform systems was used. Determination of lipid classes in the lipid extracts using silica gel thin-layer chromatography on Chromarods-SIII, with flame ionisation detection by latroscan, suggested that the lower lipid recovery by the hexane/isopropanol method may be due to a less effective extraction of the more polar lipid classes. including oxidation products, present in brown fish meal." @default.
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- W2002068942 title "Three extraction methods for determination of lipids in fish meal: Evaluation of a hexane/isopropanol method as an alternative to chloroform-based methods" @default.
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