FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2002074058> ?p ?o ?g. }

• W2002074058 endingPage "63" @default.
• W2002074058 startingPage "45" @default.
• W2002074058 abstract "Specific binding sites for testosterone have been detected in three compartments of olfactory tissue from brown and rainbow trout. Binding of3H-testosterone to the membrane fraction of olfactory tissue is of high affinity (Kd = 0.5–1.9 nM) and limited capacity (Nmax = 30–60 fmol mg+1 protein). Binding is reversible, and is eliminated by protease treatment. The membrane binding site exhibits a high degree of ligand specificity; 11β-hydroxytestosterone, 11-ketotestosterone, 17α-hydroxyprogesterone, 17α,20β-dihydroxy-4-pregnen-3-one, cortisol, and estradiol-17β all fail to displace testosterone at 20-fold excess while testosterone itself competes successfully. These attributes are consistent with the presence of specific steroid receptor proteins. Binding of testosterone within the cytosol is of moderate affinity (Kd = 9.0–23.0 nM) and high capacity (Nmax = 0.5–2.9 pmol mg+1 protein) and is more readily displaced by a number of steroid competitors than is the case for the membrane site. The rate of association and dissociation of testosterone from the cytosolic binding site is markedly more rapid than the equivalent processes in the membrane fraction. Binding of testosterone to the nuclear extract is of high affinity (Kd ∼3.0 nM) and limited capacity (Nmax ∼50 fmol mg+1 protein). There are no substantial differences between species or between sexes in the affinity or capacity of testosterone-binding sites in nuclear extract or membrane fraction. However, cytosolic testosterone-binding sites are three- to four-fold more abundant in rainbow trout than in brown trout, and female rainbow trout have more cytosolic binding sites than male rainbow trout, but a lower affinity for testosterone than male sites. Preliminary evidence supports the involvement of the membrane-associated testosterone-binding site in olfactory processes. Rainbow trout display an EOG response to testosterone at a concentration (≥ 10+9 M) which is consistent with the equilibrium dissociation constant (Kd) of the membrane-associated testosterone-binding site. Binding of3H-testosterone to the membrane-associated site shows a pH dependency which is comparable to the effects of pH on the EOG response to testosterone in intact fish. The attributes of the intracellular testosterone-binding sites are common to testosterone receptors in other fish tissues which are known androgen target tissues. This suggests that the development and/or function of salmonid olfactory tissue may be susceptible to influence by endogenous testosterone." @default.
• W2002074058 created "2016-06-24" @default.
• W2002074058 creator A5068508088 @default.
• W2002074058 creator A5079068266 @default.
• W2002074058 date "1997-01-01" @default.
• W2002074058 modified "2023-09-26" @default.
• W2002074058 title "Characterization of putative steroid receptors in the membrane, cytosol and nuclear fractions from the olfactory tissue of brown and rainbow trout" @default.
• W2002074058 cites W1049215730 @default.
• W2002074058 cites W105399818 @default.
• W2002074058 cites W1151831416 @default.
• W2002074058 cites W1867286908 @default.
• W2002074058 cites W1962989710 @default.
• W2002074058 cites W1968262020 @default.
• W2002074058 cites W1968771740 @default.
• W2002074058 cites W1982777113 @default.
• W2002074058 cites W1989250170 @default.
• W2002074058 cites W1991574617 @default.
• W2002074058 cites W1992629448 @default.
• W2002074058 cites W1994067810 @default.
• W2002074058 cites W1999836476 @default.
• W2002074058 cites W2000584233 @default.
• W2002074058 cites W2004262087 @default.
• W2002074058 cites W2009016291 @default.
• W2002074058 cites W2009637899 @default.
• W2002074058 cites W2009908154 @default.
• W2002074058 cites W2013073054 @default.
• W2002074058 cites W2016628764 @default.
• W2002074058 cites W2020738539 @default.
• W2002074058 cites W2026929739 @default.
• W2002074058 cites W2027157632 @default.
• W2002074058 cites W2028247629 @default.
• W2002074058 cites W2032937969 @default.
• W2002074058 cites W2037751256 @default.
• W2002074058 cites W2039813397 @default.
• W2002074058 cites W2040357956 @default.
• W2002074058 cites W2050995032 @default.
• W2002074058 cites W2052334474 @default.
• W2002074058 cites W2061528650 @default.
• W2002074058 cites W2065373256 @default.
• W2002074058 cites W2065852731 @default.
• W2002074058 cites W2067248617 @default.
• W2002074058 cites W2084487909 @default.
• W2002074058 cites W2085643546 @default.
• W2002074058 cites W2087972809 @default.
• W2002074058 cites W2088188704 @default.
• W2002074058 cites W2089693058 @default.
• W2002074058 cites W2101287457 @default.
• W2002074058 cites W2112916772 @default.
• W2002074058 cites W2128753406 @default.
• W2002074058 cites W2155478307 @default.
• W2002074058 cites W2329270964 @default.
• W2002074058 doi "https://doi.org/10.1007/bf00004540" @default.
• W2002074058 hasPublicationYear "1997" @default.
• W2002074058 type Work @default.
• W2002074058 sameAs 2002074058 @default.
• W2002074058 citedByCount "20" @default.
• W2002074058 countsByYear W20020740582012 @default.
• W2002074058 countsByYear W20020740582013 @default.
• W2002074058 countsByYear W20020740582014 @default.
• W2002074058 countsByYear W20020740582019 @default.
• W2002074058 countsByYear W20020740582023 @default.
• W2002074058 crossrefType "journal-article" @default.
• W2002074058 hasAuthorship W2002074058A5068508088 @default.
• W2002074058 hasAuthorship W2002074058A5079068266 @default.
• W2002074058 hasBestOaLocation W20020740582 @default.
• W2002074058 hasConcept C107824862 @default.
• W2002074058 hasConcept C126322002 @default.
• W2002074058 hasConcept C134018914 @default.
• W2002074058 hasConcept C170493617 @default.
• W2002074058 hasConcept C181199279 @default.
• W2002074058 hasConcept C2779279991 @default.
• W2002074058 hasConcept C2780902042 @default.
• W2002074058 hasConcept C2909208804 @default.
• W2002074058 hasConcept C2994167347 @default.
• W2002074058 hasConcept C505870484 @default.
• W2002074058 hasConcept C55493867 @default.
• W2002074058 hasConcept C71315377 @default.
• W2002074058 hasConcept C71924100 @default.
• W2002074058 hasConcept C74998103 @default.
• W2002074058 hasConcept C85196483 @default.
• W2002074058 hasConcept C86803240 @default.
• W2002074058 hasConcept C98539663 @default.
• W2002074058 hasConceptScore W2002074058C107824862 @default.
• W2002074058 hasConceptScore W2002074058C126322002 @default.
• W2002074058 hasConceptScore W2002074058C134018914 @default.
• W2002074058 hasConceptScore W2002074058C170493617 @default.
• W2002074058 hasConceptScore W2002074058C181199279 @default.
• W2002074058 hasConceptScore W2002074058C2779279991 @default.
• W2002074058 hasConceptScore W2002074058C2780902042 @default.
• W2002074058 hasConceptScore W2002074058C2909208804 @default.
• W2002074058 hasConceptScore W2002074058C2994167347 @default.
• W2002074058 hasConceptScore W2002074058C505870484 @default.
• W2002074058 hasConceptScore W2002074058C55493867 @default.
• W2002074058 hasConceptScore W2002074058C71315377 @default.
• W2002074058 hasConceptScore W2002074058C71924100 @default.
• W2002074058 hasConceptScore W2002074058C74998103 @default.
• W2002074058 hasConceptScore W2002074058C85196483 @default.
• W2002074058 hasConceptScore W2002074058C86803240 @default.

• next