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- W2002120697 abstract "In situ determinations of the spatial relationship between the nuclear pore and its annulus were made in ultrathin sections of African green monkey kidney and mouse 3T3 tissue culture cells, embedded as monolayers. A variety of fixation procedures, enzyme digestions, serial sections, and rotational analysis of selected images were used to analyze the structure of the pore complex. The results indicated that the annulus of each pore consists of at least two types of subunits; that it extends both into the cytoplasm and into the nucleoplasm and fits within the margins of the pore at the level of the nuclear envelope. The pore complex is hourglass-shaped since the diameter of the cytoplasmic and nuclear extensions are greater than the nuclear pore. The octagonal nature of the nuclear pore itself was confirmed. In addition, the predominant number of subannular components was also found to be eight. These observations are discussed in the light of the findings of earlier investigators and serve to clarify the structural complexity of the pore complex. On the basis of our results, which have been confirmed by three-dimensional reconstruction of the pore-annulus relationship from serial sections, we suggest that the nuclear pore complex be included among the classical cellular organelles." @default.
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- W2002120697 title "Nuclear pores: the pore-annulus relationship in thin section" @default.
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- W2002120697 doi "https://doi.org/10.1016/s0022-5320(70)90052-3" @default.
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