FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2002126149> ?p ?o ?g. }

• W2002126149 endingPage "1613" @default.
• W2002126149 startingPage "1606" @default.
• W2002126149 abstract "Background The important dust mite allergens identified to date are of molecular weights ranging from 14 to 60 kDa. Our previous protein study indicated that the 98-kDa native paramyosin in Dermatophagoides farinae mite showed IgE reactivity with 82% of the mite-sensitive asthmatic patients suggesting that it is a novel major mite allergen. This study described the isolation and characterization of the cDNA clone encoding the 98-kDa mite allergen. Methods A Dermatophagoides farinae cDNA library was constructed in lambda ZAPII vector and the library was immunoscreened with a monoclonal antibody 642. The cDNA insert was sub-cloned into M13 sequencing vector for single-stranded sequencing. The whole cDNA insert was expressed in pGEX-2T Escherichia coli expression system as a fusion protein with GST. The allergenicity of the recombinant peptides was tested by skin tests and IgE immunoassay. The IgE and IgG immunoassays were performed with sera from 20 mite-allergic patients. Results The cDNA clone Df642 was 2134 bp long, coding for a polypeptide of 711 amino acid residues. Protein sequence analysis and alignment confirmed that the deduced polypeptide is a mite paramyosin which is truncated slightly at the N- and C-terminuses. In vivo skin tests and in vitro IgE-binding study showed that 62% (13/21) and 50% (10/20) of the mite-sensitive asthmatic patients reacted positively with the recombinant Dermatophagoides farinae paramyosin, respectively. Conclusion The study indicated that 98-kDa mite paramyosin is an important allergen." @default.
• W2002126149 created "2016-06-24" @default.
• W2002126149 creator A5008451903 @default.
• W2002126149 creator A5012104141 @default.
• W2002126149 creator A5040906209 @default.
• W2002126149 creator A5043810951 @default.
• W2002126149 creator A5052723204 @default.
• W2002126149 creator A5069512323 @default.
• W2002126149 creator A5071953681 @default.
• W2002126149 creator A5076221819 @default.
• W2002126149 date "1999-12-01" @default.
• W2002126149 modified "2023-10-08" @default.
• W2002126149 title "Sequence analysis and expression of a cDNA clone encoding a 98-kDa allergen in<i>Dermatophagoides farinae</i>" @default.
• W2002126149 cites W1502627693 @default.
• W2002126149 cites W1584943483 @default.
• W2002126149 cites W1601687245 @default.
• W2002126149 cites W1963529285 @default.
• W2002126149 cites W1964229883 @default.
• W2002126149 cites W1964841886 @default.
• W2002126149 cites W1965231243 @default.
• W2002126149 cites W2029693770 @default.
• W2002126149 cites W2030164644 @default.
• W2002126149 cites W2036759647 @default.
• W2002126149 cites W2043180019 @default.
• W2002126149 cites W2046519736 @default.
• W2002126149 cites W2056472396 @default.
• W2002126149 cites W2057636913 @default.
• W2002126149 cites W2059359155 @default.
• W2002126149 cites W2060333964 @default.
• W2002126149 cites W2064793834 @default.
• W2002126149 cites W2066203499 @default.
• W2002126149 cites W2067791817 @default.
• W2002126149 cites W2068446924 @default.
• W2002126149 cites W2076446879 @default.
• W2002126149 cites W2106882534 @default.
• W2002126149 cites W2114757629 @default.
• W2002126149 cites W2138270253 @default.
• W2002126149 cites W2158473123 @default.
• W2002126149 cites W2169159326 @default.
• W2002126149 cites W2169581200 @default.
• W2002126149 cites W2172159050 @default.
• W2002126149 cites W86083456 @default.
• W2002126149 doi "https://doi.org/10.1046/j.1365-2222.1999.00603.x" @default.
• W2002126149 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10594536" @default.
• W2002126149 hasPublicationYear "1999" @default.
• W2002126149 type Work @default.
• W2002126149 sameAs 2002126149 @default.
• W2002126149 citedByCount "28" @default.
• W2002126149 countsByYear W20021261492012 @default.
• W2002126149 countsByYear W20021261492013 @default.
• W2002126149 countsByYear W20021261492014 @default.
• W2002126149 countsByYear W20021261492022 @default.
• W2002126149 countsByYear W20021261492023 @default.
• W2002126149 crossrefType "journal-article" @default.
• W2002126149 hasAuthorship W2002126149A5008451903 @default.
• W2002126149 hasAuthorship W2002126149A5012104141 @default.
• W2002126149 hasAuthorship W2002126149A5040906209 @default.
• W2002126149 hasAuthorship W2002126149A5043810951 @default.
• W2002126149 hasAuthorship W2002126149A5052723204 @default.
• W2002126149 hasAuthorship W2002126149A5069512323 @default.
• W2002126149 hasAuthorship W2002126149A5071953681 @default.
• W2002126149 hasAuthorship W2002126149A5076221819 @default.
• W2002126149 hasConcept C104317684 @default.
• W2002126149 hasConcept C141105273 @default.
• W2002126149 hasConcept C153911025 @default.
• W2002126149 hasConcept C159654299 @default.
• W2002126149 hasConcept C167625842 @default.
• W2002126149 hasConcept C187882448 @default.
• W2002126149 hasConcept C189482833 @default.
• W2002126149 hasConcept C203014093 @default.
• W2002126149 hasConcept C207480886 @default.
• W2002126149 hasConcept C2778166374 @default.
• W2002126149 hasConcept C2779711295 @default.
• W2002126149 hasConcept C2780510475 @default.
• W2002126149 hasConcept C40767141 @default.
• W2002126149 hasConcept C55493867 @default.
• W2002126149 hasConcept C59822182 @default.
• W2002126149 hasConcept C81089528 @default.
• W2002126149 hasConcept C86803240 @default.
• W2002126149 hasConceptScore W2002126149C104317684 @default.
• W2002126149 hasConceptScore W2002126149C141105273 @default.
• W2002126149 hasConceptScore W2002126149C153911025 @default.
• W2002126149 hasConceptScore W2002126149C159654299 @default.
• W2002126149 hasConceptScore W2002126149C167625842 @default.
• W2002126149 hasConceptScore W2002126149C187882448 @default.
• W2002126149 hasConceptScore W2002126149C189482833 @default.
• W2002126149 hasConceptScore W2002126149C203014093 @default.
• W2002126149 hasConceptScore W2002126149C207480886 @default.
• W2002126149 hasConceptScore W2002126149C2778166374 @default.
• W2002126149 hasConceptScore W2002126149C2779711295 @default.
• W2002126149 hasConceptScore W2002126149C2780510475 @default.
• W2002126149 hasConceptScore W2002126149C40767141 @default.
• W2002126149 hasConceptScore W2002126149C55493867 @default.
• W2002126149 hasConceptScore W2002126149C59822182 @default.
• W2002126149 hasConceptScore W2002126149C81089528 @default.
• W2002126149 hasConceptScore W2002126149C86803240 @default.
• W2002126149 hasIssue "12" @default.
• W2002126149 hasLocation W20021261491 @default.

• next