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- W2002126500 abstract "Wheat ribulose-1,5-diphosphate carboxylase purified to homogeneity had a MW of 540 000, sedimentation coefficient (S20, W) of 18.5 S, apparent diffusion constant (Dapp) of 3.07 × 10−7 cm2/sec, Stoke's radius 5.44 nm, and fractional ratio of 1.17. Electron microscopy revealed particles of 10–12 nm diameter. The enzyme was dissociated by sodium dodecyl sulphate into two subunits of MW 53 000 (S20, W = 3.0 S) and 13 500 (S20, W = 1.7 S). The total amino acid residues in the large and small subunits were 481 and 117, respectively. Tryptic peptide maps of the two subunits confirmed the estimated numbers of Arg and Lys residues. Although the amino acid pattern of the large subunit closely resembled that from barley, rather than that for spinach, beet or tobacco, the pattern of the small subunit was markedly different from those of all the other species." @default.
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- W2002126500 date "1979-01-01" @default.
- W2002126500 modified "2023-09-24" @default.
- W2002126500 title "Isolation and characterization of wheat ribulose-1,5-diphosphate carboxylase" @default.
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- W2002126500 doi "https://doi.org/10.1016/s0031-9422(00)84260-0" @default.
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