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• W2002137466 abstract "ABSTRACT Haemophilus somnus strain 649 was found to acquire iron from ovine, bovine, and goat transferrins (Tfs). Expression of Tf receptors, as evaluated by solid-phase binding assays, required the organisms to be grown under iron-restricted conditions in the presence of Tf. Competition binding assays revealed the presence of two distinct Tf-binding receptor systems, one specific for bovine Tf and the other capable of binding all three ruminant Tfs. Affinity isolation procedures using total membranes yielded three putative bovine Tf-binding polypeptides and one putative ovine and goat Tf-binding polypeptide. PCR amplification followed by DNA sequence analyses revealed that H. somnus strain 649 possesses genes that encode a bipartite TbpA-TbpB receptor along with a homolog of the Histophilus ovis single-component TbpA receptor. Expression of TbpB and the single-component TbpA would appear to be subject to a form of phase variation involving homopolymeric nucleotide tracts within the structural genes." @default.
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• W2002137466 date "2004-07-01" @default.
• W2002137466 modified "2023-09-26" @default.
• W2002137466 title "<i>Haemophilus somnus</i> Possesses Two Systems for Acquisition of Transferrin-Bound Iron" @default.
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• W2002137466 doi "https://doi.org/10.1128/jb.186.13.4407-4411.2004" @default.
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