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- W2002138450 abstract "The changes in phosphate metabolism induced in yeast by transition from fermentation to respiration have been studied. Orthophosphate added to respiring or fermenting yeast suspensions as Na2HP32O4 is rapidly resorbed and incorporated into adenosine triphosphate (ATP) and other acid-labile fractions. During fermentation, the specific activity of the orthophosphate is higher than that of ATP. This is thought to be mainly due to a heterogeneity in the intracellular orthophosphate. In respiring yeast, pyrophosphate is formed. The specific activity of this pyrophosphate is very high when the cells are maintained from the start of the experiment under aerobic conditions. When respiration follows a prior period of fermentation lasting 30–60 min., an accumulation of lowly labeled pyrophosphate occurs. Concurrently an acidinsoluble phosphate fraction is mobilized. As indicated by labeling relations, this fraction may be an intermediary in the pathway between orthophosphate and pyrophosphate. The possible role of dinucleotides in primary aerobic phosphorylation is reviewed and it is shown that diphosphopyridine nucleotide (DPN) undergoes a temporary resynthesis in yeast during the first 5–6 hr. of respiration. The question whether this phenomenon may be regarded as a secondary consequence of an enzymatic adaptation which involves pyrophosphate accumulation is discussed." @default.
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- W2002138450 date "1951-03-01" @default.
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- W2002138450 title "Changes in phosphate metabolism induced in yeast by transition from fermentation to respiration" @default.
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- W2002138450 doi "https://doi.org/10.1016/0003-9861(51)90190-7" @default.
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