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• W2002144258 abstract "ObjectiveTo determine the effects of interleukin (IL)-6 and oncostatin M (OSM) added separately or in combination with IL-1β on human osteoarthritic (OA) chondrocytes in alginate beads.DesignHuman chondrocytes were isolated from OA cartilage and cultured in alginate beads for 12 days, in the absence or in the presence of increasing amounts of IL-6 (20–500 ng/ml) with its soluble receptor or OSM (0.1–10 ng/ml) and with or without IL-1β (1.7 ng/ml). Aggrecan (AGG), transforming growth factor-β1 (TGF-β1), stromelysin-1 [matrix metalloprotease (MMP)-3], tissue inhibitor of metalloproteinases-1 (TIMP-1), macrophage inflammatory protein-1 beta (MIP-1β), IL-6 and IL-8 productions were assayed by specific enzyme amplified sensitivity immunoassays. Prostaglandin (PG)E2 was measured by a specific radioimmunoassay and nitrite (NO2−) by a spectrophotometric method based upon the Griess reaction.ResultsOSM, but not IL-6, decreased basal AGG and TGF-β1 synthesis. Although IL-6 stimulated basal TIMP-1 production, it did not significantly modify MMP-3/TIMP-1 ratio. In contrast, 10 ng/ml OSM highly increased TIMP-1 production, and decreased by half the ratio MMP-3/TIMP-1. IL-1β highly stimulated NO, IL-8, IL-6, MIP-1β and PGE2 synthesis but decreased AGG and TGF-β1 production. Neither IL-6 nor OSM modulated IL-1β-inhibitory effect on AGG production. IL-6, but not OSM, reversed IL-1β-induced TGF-β1 inhibition. At 1–10 ng/ml, OSM significantly decreased IL-1β-stimulated IL-8, MIP-1β, PGE2 and NO production but amplified IL-1β stimulating effect on IL-6 production. IL-6 had no effect on these parameters.ConclusionsOSM and IL-6, two glycoprotein 130 binding cytokines, show different activity profiles on OA chondrocytes, indicating that these cytokines could play different roles in the OA disease process." @default.
• W2002144258 created "2016-06-24" @default.
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• W2002144258 date "2004-10-01" @default.
• W2002144258 modified "2023-10-16" @default.
• W2002144258 title "Differential regulation of chondrocyte metabolism by oncostatin M and interleukin-6" @default.
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• W2002144258 doi "https://doi.org/10.1016/j.joca.2004.06.011" @default.
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