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- W2002144270 abstract "Bacteria perform chemotaxis by regulation of their tumbling frequency. Quantitatively, the frequency of tumbling is regulated by the convolution of the history of the attractant detections with the impulse response function (kernel), which measures the response of the bacterium to an impulse stimulus. At the molecular level, the response is shaped by the molecular processes of (de)phosphorylation and (de)methylation. Experiments to measure the chemotactic response function are currently realized using the classical tethering technique (Silverman and Simon, 1974) where flagella are tethered to a glass slide via a flagellin antibody . Flagella cannot rotate, whilst counter-rotation of the bacterium is visible at the microscope. This occurs when only a single flagellum is tethered and E. coli is pre-treated to reduce it to a mono-flagellated state. The statistics of rotations clock-wise or anti-clockwise (corresponding to runs and tumbles) are thus measured in response to different stimuli. We have developed a novel inference method to measure the chemotactic response function [1]. Bacteria are introduced in a 300-μm deep channel where a static and homogeneous gradient of a chemical is established. The inference is performed on the recorded trajectories of bacteria swimming in chemoattractant (or repellent) and is thus non-intrusive. Only the concentration of chemoattractant (repellent) and the times of tumble are necessary to extract the response function. Using this method we have characterized the chemotactic response of E. coli for numerous attractants and repellents. We studied the modification of the chemotactic response with the concentration of attractant and repellent, with the magnitude of the gradients and with gene deletions. Finally, we analyzed the modification of the response function due to the loss of adaptation to one attractant induced by a second attractant. [1] Voisinne et al, submitted to Cell" @default.
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- W2002144270 date "2011-02-01" @default.
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- W2002144270 title "Non Invasive Inference of Chemotaxis Responses from Bacterial Trajectories" @default.
- W2002144270 doi "https://doi.org/10.1016/j.bpj.2010.12.3011" @default.
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