Matches in SemOpenAlex for { <https://semopenalex.org/work/W2002150714> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2002150714 endingPage "393" @default.
- W2002150714 startingPage "389" @default.
- W2002150714 abstract "A rapid real-time polymerase chain reaction (PCR) assay using molecular beacons has been developed for the simultaneous detection of wild-type and mutant strains of cytomegaloviruses (CMV) with respect to codon 460 of the UL97 gene has been developed. The molecular beacons were designed to complement the wild-type codon 460 or the mutant sequence arising from a single base-pair difference (point mutation). Discrimination between wild-type and mutant templates was demonstrated as the beacons did not generate fluorescence with their respective mismatch targets but only with those that they were designed to perfectly anneal with. Samples that harbor mixed populations of CMV could also be readily recognized. Applied to a small number of clinical samples, the retrospective screening by this assay are in general concordance with that obtained by PCR-RFLP. Using molecular beacons strategy, codon 460 mutation was detected in ten out o the total number of 40 samples, whereas the latter method identified nine samples as containing the mutation. The discrepant result arose from the genotyping of one clinical sample as mixed (containing both wild-type and mutant CMV strains) by molecular beacons but as wild-type by PCR-RFLP, suggesting that this real-time strategy is possibly more sensitive for mutation analysis." @default.
- W2002150714 created "2016-06-24" @default.
- W2002150714 creator A5012629866 @default.
- W2002150714 creator A5040570732 @default.
- W2002150714 creator A5046588695 @default.
- W2002150714 creator A5089771834 @default.
- W2002150714 date "2005-12-01" @default.
- W2002150714 modified "2023-10-18" @default.
- W2002150714 title "Rapid detection of codon 460 mutations in the UL97 gene of ganciclovir-resistant cytomegalovirus clinical isolates by real-time PCR using molecular beacons" @default.
- W2002150714 cites W1893350708 @default.
- W2002150714 cites W2026710381 @default.
- W2002150714 cites W2026999862 @default.
- W2002150714 cites W2036876068 @default.
- W2002150714 cites W2042682894 @default.
- W2002150714 cites W2048450381 @default.
- W2002150714 cites W2054092356 @default.
- W2002150714 cites W2066900738 @default.
- W2002150714 cites W2071003885 @default.
- W2002150714 cites W2090222270 @default.
- W2002150714 cites W2100187217 @default.
- W2002150714 cites W2100869063 @default.
- W2002150714 cites W2108634318 @default.
- W2002150714 cites W2124847031 @default.
- W2002150714 cites W2129127406 @default.
- W2002150714 cites W2140722952 @default.
- W2002150714 cites W2167351117 @default.
- W2002150714 cites W4234856234 @default.
- W2002150714 doi "https://doi.org/10.1016/j.mcp.2005.06.008" @default.
- W2002150714 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16098713" @default.
- W2002150714 hasPublicationYear "2005" @default.
- W2002150714 type Work @default.
- W2002150714 sameAs 2002150714 @default.
- W2002150714 citedByCount "23" @default.
- W2002150714 countsByYear W20021507142012 @default.
- W2002150714 countsByYear W20021507142013 @default.
- W2002150714 countsByYear W20021507142014 @default.
- W2002150714 countsByYear W20021507142015 @default.
- W2002150714 countsByYear W20021507142016 @default.
- W2002150714 crossrefType "journal-article" @default.
- W2002150714 hasAuthorship W2002150714A5012629866 @default.
- W2002150714 hasAuthorship W2002150714A5040570732 @default.
- W2002150714 hasAuthorship W2002150714A5046588695 @default.
- W2002150714 hasAuthorship W2002150714A5089771834 @default.
- W2002150714 hasConcept C104317684 @default.
- W2002150714 hasConcept C119203544 @default.
- W2002150714 hasConcept C129312508 @default.
- W2002150714 hasConcept C135763542 @default.
- W2002150714 hasConcept C143065580 @default.
- W2002150714 hasConcept C153911025 @default.
- W2002150714 hasConcept C159047783 @default.
- W2002150714 hasConcept C168007829 @default.
- W2002150714 hasConcept C176944494 @default.
- W2002150714 hasConcept C207583985 @default.
- W2002150714 hasConcept C31467283 @default.
- W2002150714 hasConcept C49105822 @default.
- W2002150714 hasConcept C501734568 @default.
- W2002150714 hasConcept C54355233 @default.
- W2002150714 hasConcept C86803240 @default.
- W2002150714 hasConceptScore W2002150714C104317684 @default.
- W2002150714 hasConceptScore W2002150714C119203544 @default.
- W2002150714 hasConceptScore W2002150714C129312508 @default.
- W2002150714 hasConceptScore W2002150714C135763542 @default.
- W2002150714 hasConceptScore W2002150714C143065580 @default.
- W2002150714 hasConceptScore W2002150714C153911025 @default.
- W2002150714 hasConceptScore W2002150714C159047783 @default.
- W2002150714 hasConceptScore W2002150714C168007829 @default.
- W2002150714 hasConceptScore W2002150714C176944494 @default.
- W2002150714 hasConceptScore W2002150714C207583985 @default.
- W2002150714 hasConceptScore W2002150714C31467283 @default.
- W2002150714 hasConceptScore W2002150714C49105822 @default.
- W2002150714 hasConceptScore W2002150714C501734568 @default.
- W2002150714 hasConceptScore W2002150714C54355233 @default.
- W2002150714 hasConceptScore W2002150714C86803240 @default.
- W2002150714 hasIssue "6" @default.
- W2002150714 hasLocation W20021507141 @default.
- W2002150714 hasLocation W20021507142 @default.
- W2002150714 hasOpenAccess W2002150714 @default.
- W2002150714 hasPrimaryLocation W20021507141 @default.
- W2002150714 hasRelatedWork W101124211 @default.
- W2002150714 hasRelatedWork W1527128310 @default.
- W2002150714 hasRelatedWork W1982821581 @default.
- W2002150714 hasRelatedWork W1986920602 @default.
- W2002150714 hasRelatedWork W2001968092 @default.
- W2002150714 hasRelatedWork W2013326849 @default.
- W2002150714 hasRelatedWork W2060397969 @default.
- W2002150714 hasRelatedWork W2349294463 @default.
- W2002150714 hasRelatedWork W2379610755 @default.
- W2002150714 hasRelatedWork W4284892098 @default.
- W2002150714 hasVolume "19" @default.
- W2002150714 isParatext "false" @default.
- W2002150714 isRetracted "false" @default.
- W2002150714 magId "2002150714" @default.
- W2002150714 workType "article" @default.