FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2002155693> ?p ?o ?g. }

• W2002155693 endingPage "72" @default.
• W2002155693 startingPage "68" @default.
• W2002155693 abstract "The NRT1/PTR family of proton-coupled transporters are responsible for nitrogen assimilation in eukaryotes and bacteria through the uptake of peptides. However, in most plant species members of this family have evolved to transport nitrate as well as additional secondary metabolites and hormones. In response to falling nitrate levels, NRT1.1 is phosphorylated on an intracellular threonine that switches the transporter from a low-affinity to high-affinity state. Here we present both the apo and nitrate-bound crystal structures of Arabidopsis thaliana NRT1.1, which together with in vitro binding and transport data identify a key role for His 356 in nitrate binding. Our data support a model whereby phosphorylation increases structural flexibility and in turn the rate of transport. Comparison with peptide transporters further reveals how the NRT1/PTR family has evolved to recognize diverse nitrogenous ligands, while maintaining elements of a conserved coupling mechanism within this superfamily of nutrient transporters. In Arabidopsis thaliana the phosphorylation state of the ‘dual affinity’ transporter, NRT1.1, allows the uptake of nitrate over a wide concentration range; the crystal structure and molecular basis for this is described in this study. Soil levels of nitrate, a primary nutrient for plant growth, can vary dramatically. Plants therefore need a versatile mechanism for obtaining nitrate from the environment. In the model plant Arabidopsis thaliana, the dual-affinity transporter NRT1.1 can take up nitrate across a broad range of concentrations, switching from low- to high-affinity mode according to the phosphorylation status of a key threonine residue. Two studies published in this issue of Nature describe the crystal structures of full-length NRT1.1, providing insights into how this post-translational modification switches the transporter between the low-affinity and high-affinity states." @default.
• W2002155693 created "2016-06-24" @default.
• W2002155693 creator A5017714497 @default.
• W2002155693 creator A5022968869 @default.
• W2002155693 date "2014-02-26" @default.
• W2002155693 modified "2023-10-06" @default.
• W2002155693 title "Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1" @default.
• W2002155693 cites W1538833285 @default.
• W2002155693 cites W1964566953 @default.
• W2002155693 cites W1987307039 @default.
• W2002155693 cites W1991764055 @default.
• W2002155693 cites W1998884774 @default.
• W2002155693 cites W2000350633 @default.
• W2002155693 cites W2002158823 @default.
• W2002155693 cites W2004107805 @default.
• W2002155693 cites W2006922696 @default.
• W2002155693 cites W2009172990 @default.
• W2002155693 cites W2013083986 @default.
• W2002155693 cites W2020915126 @default.
• W2002155693 cites W2024056631 @default.
• W2002155693 cites W2024112041 @default.
• W2002155693 cites W2028833004 @default.
• W2002155693 cites W2037823802 @default.
• W2002155693 cites W2038749716 @default.
• W2002155693 cites W2047904157 @default.
• W2002155693 cites W2052966154 @default.
• W2002155693 cites W2055286572 @default.
• W2002155693 cites W2061068248 @default.
• W2002155693 cites W2061110447 @default.
• W2002155693 cites W2061847624 @default.
• W2002155693 cites W2089656274 @default.
• W2002155693 cites W2101446086 @default.
• W2002155693 cites W2108098442 @default.
• W2002155693 cites W2108558096 @default.
• W2002155693 cites W2109021353 @default.
• W2002155693 cites W2119013150 @default.
• W2002155693 cites W2119362018 @default.
• W2002155693 cites W2120817955 @default.
• W2002155693 cites W2121357833 @default.
• W2002155693 cites W2122994276 @default.
• W2002155693 cites W2126289782 @default.
• W2002155693 cites W2126890802 @default.
• W2002155693 cites W2127594598 @default.
• W2002155693 cites W2134216529 @default.
• W2002155693 cites W2139565537 @default.
• W2002155693 cites W2140660100 @default.
• W2002155693 cites W2146103350 @default.
• W2002155693 cites W2147496515 @default.
• W2002155693 cites W2148047088 @default.
• W2002155693 cites W2152976977 @default.
• W2002155693 cites W2154915448 @default.
• W2002155693 cites W2155682217 @default.
• W2002155693 cites W2159598300 @default.
• W2002155693 cites W2162384798 @default.
• W2002155693 cites W2164382089 @default.
• W2002155693 cites W2167536156 @default.
• W2002155693 cites W2179412208 @default.
• W2002155693 cites W3032609405 @default.
• W2002155693 cites W4248872320 @default.
• W2002155693 doi "https://doi.org/10.1038/nature13116" @default.
• W2002155693 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3982047" @default.
• W2002155693 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24572366" @default.
• W2002155693 hasPublicationYear "2014" @default.
• W2002155693 type Work @default.
• W2002155693 sameAs 2002155693 @default.
• W2002155693 citedByCount "298" @default.
• W2002155693 countsByYear W20021556932014 @default.
• W2002155693 countsByYear W20021556932015 @default.
• W2002155693 countsByYear W20021556932016 @default.
• W2002155693 countsByYear W20021556932017 @default.
• W2002155693 countsByYear W20021556932018 @default.
• W2002155693 countsByYear W20021556932019 @default.
• W2002155693 countsByYear W20021556932020 @default.
• W2002155693 countsByYear W20021556932021 @default.
• W2002155693 countsByYear W20021556932022 @default.
• W2002155693 countsByYear W20021556932023 @default.
• W2002155693 crossrefType "journal-article" @default.
• W2002155693 hasAuthorship W2002155693A5017714497 @default.
• W2002155693 hasAuthorship W2002155693A5022968869 @default.
• W2002155693 hasBestOaLocation W20021556932 @default.
• W2002155693 hasConcept C104317684 @default.
• W2002155693 hasConcept C11960822 @default.
• W2002155693 hasConcept C143065580 @default.
• W2002155693 hasConcept C149011108 @default.
• W2002155693 hasConcept C178790620 @default.
• W2002155693 hasConcept C185592680 @default.
• W2002155693 hasConcept C2775880066 @default.
• W2002155693 hasConcept C2775988993 @default.
• W2002155693 hasConcept C2776384668 @default.
• W2002155693 hasConcept C2776414213 @default.
• W2002155693 hasConcept C2779491563 @default.
• W2002155693 hasConcept C3119751 @default.
• W2002155693 hasConcept C55493867 @default.
• W2002155693 hasConceptScore W2002155693C104317684 @default.
• W2002155693 hasConceptScore W2002155693C11960822 @default.
• W2002155693 hasConceptScore W2002155693C143065580 @default.
• W2002155693 hasConceptScore W2002155693C149011108 @default.
• W2002155693 hasConceptScore W2002155693C178790620 @default.

• next