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- W2002159558 abstract "Abstract Bovine chromaffin cells possess several types of Ca 2+ channels, and influx of Ca 2+ is known to trigger secretion. However, discrepant information about the relative importance of the individual subtypes in secretion has been reported. We used whole‐cell patch‐clamp measurements in isolated cells in culture combined with fura‐2 microfluorimetry and pharmacological manipulation to determine the dependence of secretion on different types of Ca 2+ channels. We stimulated cells with relatively long depolarizing voltage‐clamp pulses in a medium containing 60 m m CaCl 2 . We found that, within a certain range of pulse parameters, secretion as measured by membrane capacitance changes was mainly determined by the total cumulative charge of Ca 2+ inflow and the basal [Ca 2+ ] level preceding a stimulus. Blocking or reducing the contribution of specific types of Ca 2+ channels using either 20 μ m nifedipine plus 10 μ m nimodipine or 1 μ m ωCTxGVIA (omega‐conotoxin GVIA) or 2 μ m ωCTxMVIIC (omega‐conotoxin MVIIC) reduced secretion in proportion to Ca 2+ charge, irrespective of the toxin used. We conclude that for long‐duration stimuli, which release a large fraction of the readily releasable pool of vesicles, it is not so important through which type of channels Ca 2+ enters the cell. Release is determined by the total amount of Ca 2+ entering and by the filling state of the readily releasable pool, which depends on basal [Ca 2+ ] before the stimulus. This result does not preclude that other stimulation patterns may lead to responses in which subtype specificity of Ca 2+ channels matters." @default.
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- W2002159558 date "1999-08-01" @default.
- W2002159558 modified "2023-10-03" @default.
- W2002159558 title "Different types of calcium channels and secretion from bovine chromaffin cells" @default.
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- W2002159558 doi "https://doi.org/10.1046/j.1460-9568.1999.00707.x" @default.
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