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- W2002160920 abstract "Immunochemical studies often anti-LH-RH antisera induced by four different immunogens in rabbits are reported. The apparent association constants of the hapten-antibody complexes ranged from 1.3 × 108 to 4.3 × 1011M−1. The specificity was investigated by testing cross-reaction of twenty LH-RH fragments in a LH-RH radioimmunoassay system. The antisera raised by the synthetic decapeptide linked to bovine serum albumin by carbodiimide or bisdiazotized benzidine showed similar C-terminal specificities. The 3–10 LH-RH sequence was the immunodominant group of the antigenic determinant. Opposite results were observed with an antiserum elicited by charcoal-adsorbed LH-RH. This antiserum recognized an N-terminal antigenic determinant encompassing at least the 1–7 sequence. One of the antisera produced by injection of an azoderivative of LH-RH coupled to BSA by carbodiimide specifically recognized the entire decapeptide." @default.
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- W2002160920 date "1978-01-01" @default.
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- W2002160920 title "Specificity of anti-LH-RH antisera induced by different immunogens" @default.
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- W2002160920 doi "https://doi.org/10.1016/0161-5890(78)90026-3" @default.
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