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• W2002161618 abstract "The hemoglobin-oxygen equilibrium curve and red cell 2,3-diphosphoglycerate values were measured in 29 infants before and after exchange transfusions for hyperbilirubinemia. In 12 infants the blood used for exchange was 4 to 5 days old (Group 1), while in the other 10 the blood was used within 24 hours of collection in acid-citrate-dextrose. The mean P50 and the mean red cell 2,3-diphosphoglycerate were similar in both groups prior to exchange transfusion and averaged 20.0 mm. Hg and 4,250 mμm per milliliter of red blood cells, respectively. In the infants in Group 1 the mean postexchange P50 fell to 17.1, rose to 23.0 at 24 hours, and was 27.9 mm. Hg 5 days later. The 2,3-diphosphoglycerate values at these times averaged 1.6, 3.1, and 6.5 mmoles per milliliter of red blood cells. In contrast, in the infants in Group 2, the P50 immediately after exchange transfusion had risen to 23.1, was 25.9 at 24 hours, and was 28.1 five days after exchange. The 2,3-diphosphoglycerate values at these times averaged 3.5, 4.9, and 6.5 mmoles per milliliter of red blood cells. These studies illustrate that while blood less than 5 days of age is generally considered to be suitable for exchange transfusions, considerable differences exist between blood of one and 5 days of age with respect to oxygen affinity. It is suggested that only truly fresh blood be employed for exchange transfusion in any severely ill infant. The hemoglobin-oxygen equilibrium curve and red cell 2,3-diphosphoglycerate values were measured in 29 infants before and after exchange transfusions for hyperbilirubinemia. In 12 infants the blood used for exchange was 4 to 5 days old (Group 1), while in the other 10 the blood was used within 24 hours of collection in acid-citrate-dextrose. The mean P50 and the mean red cell 2,3-diphosphoglycerate were similar in both groups prior to exchange transfusion and averaged 20.0 mm. Hg and 4,250 mμm per milliliter of red blood cells, respectively. In the infants in Group 1 the mean postexchange P50 fell to 17.1, rose to 23.0 at 24 hours, and was 27.9 mm. Hg 5 days later. The 2,3-diphosphoglycerate values at these times averaged 1.6, 3.1, and 6.5 mmoles per milliliter of red blood cells. In contrast, in the infants in Group 2, the P50 immediately after exchange transfusion had risen to 23.1, was 25.9 at 24 hours, and was 28.1 five days after exchange. The 2,3-diphosphoglycerate values at these times averaged 3.5, 4.9, and 6.5 mmoles per milliliter of red blood cells. These studies illustrate that while blood less than 5 days of age is generally considered to be suitable for exchange transfusions, considerable differences exist between blood of one and 5 days of age with respect to oxygen affinity. It is suggested that only truly fresh blood be employed for exchange transfusion in any severely ill infant." @default.
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• W2002161618 title "Exchange transfusion in the newborn infant with fresh and “old” blood: The role of storage on 2,3-diphosphoglycerate, hemoglobin-oxygen affinity, and oxygen release" @default.
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• W2002161618 doi "https://doi.org/10.1016/s0022-3476(71)80181-6" @default.
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