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- W2002166955 abstract "It has previously been shown that concentrated solutions of guanidine hydrochloride (GHCI) in an SSC medium (0.15 M NaCl; 0.015 M sodium citrate) at room temperature do not cause irreversible denaturation [l] to DNA but severely denature proteins [2] Denatured protein components of cell nuclei, viruses, and bacterial particles are soluble in this medium. By separating DNA and protein components by gel filtration [3] , followed by an additional purification (if necessary) on a methylated albumin column (MAC) [4], highly polymerous, pure DNA can be obtained. In this paper, certain physico-chemical characteristics of nuclear thymus DNA isolated by gel filtration in 4 M GHCl are compared with the properties of DNA prepared according to Kay [5]." @default.
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- W2002166955 date "1971-04-30" @default.
- W2002166955 modified "2023-10-03" @default.
- W2002166955 title "Highly polymerous nuclear DNA from calf thymus isolated by gel filtration in 4 M guanidine hydrochloride" @default.
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- W2002166955 doi "https://doi.org/10.1016/0014-5793(71)80093-5" @default.
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