Matches in SemOpenAlex for { <https://semopenalex.org/work/W2002168701> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W2002168701 endingPage "1448" @default.
- W2002168701 startingPage "1446" @default.
- W2002168701 abstract "Mammalian glutamate dehydrogenase (GDH) is a housekeeping mitochondrial enzyme (hGDH1 in the human) that catalyses the reversible inter-conversion of glutamate to α-ketoglutarate and ammonia, thus interconnecting amino acid and carbohydrate metabolism. It displays an energy sensing mechanism, which permits enzyme activation under low cellular energy states. As GDH is at the crossroads of important metabolic pathways, a tight control of its activity is essential. Indeed, to fulfill its role in metabolism and cellular energetics, mammalian GDH has evolved into a highly regulated enzyme subject to allosteric modulation by diverse compounds. The recent emergence (<23 million years ago) in apes and humans of a hGDH2 isoenzyme with distinct regulatory properties, as well as, the detection of gain-of-function variants in hGDH1 and hGDH2 that affect the nervous system, have introduced additional complexities. The properties of the two highly homologous human GDHs were studied using purified recombinant hGDH1 and hGDH2 obtained by expression of the corresponding cDNAs in Sf21 cells. Results showed that, in contrast to hGDH1 that maintains substantial basal activity (35–40% of its maximal capacity), hGDH2 displays low basal activity (3–8% of maximal) that is remarkably responsive to activation by rising levels of ADP and/or l-leucine. This is primarily due to the Arg443Ser evolutionary change, which also made hGDH2 markedly sensitive to estrogens and neuroleptic drugs. In contrast to hGDH1, which is subject to potent GTP inhibition, hGDH2 has dissociated its function from this energy switch, being able to metabolize glutamate even when the Krebs cycle generates GTP levels sufficient to inactivate the housekeeping hGDH1. Our data also show that spermidine, a polyamine thought to reduce oxidative stress and to prolong survival, and EGCG, a green tea polyphenol, inhibit hGDH2 at lower concentrations than hGDH1. The implications of these findings in nerve tissue biology are discussed." @default.
- W2002168701 created "2016-06-24" @default.
- W2002168701 creator A5027558918 @default.
- W2002168701 creator A5028107395 @default.
- W2002168701 creator A5076799723 @default.
- W2002168701 date "1982-04-01" @default.
- W2002168701 modified "2023-09-27" @default.
- W2002168701 title "Lack of effect on glutamate dehydrogenase activity after in vivo administration of pharmacological doses of haloperidol" @default.
- W2002168701 cites W1684853394 @default.
- W2002168701 cites W1775749144 @default.
- W2002168701 cites W1987812528 @default.
- W2002168701 cites W2024104618 @default.
- W2002168701 cites W2081790032 @default.
- W2002168701 cites W2082547683 @default.
- W2002168701 cites W2100346216 @default.
- W2002168701 cites W2414976887 @default.
- W2002168701 doi "https://doi.org/10.1016/0006-2952(82)90043-0" @default.
- W2002168701 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7092934" @default.
- W2002168701 hasPublicationYear "1982" @default.
- W2002168701 type Work @default.
- W2002168701 sameAs 2002168701 @default.
- W2002168701 citedByCount "3" @default.
- W2002168701 countsByYear W20021687012012 @default.
- W2002168701 countsByYear W20021687012013 @default.
- W2002168701 crossrefType "journal-article" @default.
- W2002168701 hasAuthorship W2002168701A5027558918 @default.
- W2002168701 hasAuthorship W2002168701A5028107395 @default.
- W2002168701 hasAuthorship W2002168701A5076799723 @default.
- W2002168701 hasConcept C118716 @default.
- W2002168701 hasConcept C125209161 @default.
- W2002168701 hasConcept C165135838 @default.
- W2002168701 hasConcept C166342909 @default.
- W2002168701 hasConcept C170493617 @default.
- W2002168701 hasConcept C181199279 @default.
- W2002168701 hasConcept C2776580952 @default.
- W2002168701 hasConcept C2777202666 @default.
- W2002168701 hasConcept C515207424 @default.
- W2002168701 hasConcept C55493867 @default.
- W2002168701 hasConcept C61174792 @default.
- W2002168701 hasConcept C62231903 @default.
- W2002168701 hasConcept C86803240 @default.
- W2002168701 hasConceptScore W2002168701C118716 @default.
- W2002168701 hasConceptScore W2002168701C125209161 @default.
- W2002168701 hasConceptScore W2002168701C165135838 @default.
- W2002168701 hasConceptScore W2002168701C166342909 @default.
- W2002168701 hasConceptScore W2002168701C170493617 @default.
- W2002168701 hasConceptScore W2002168701C181199279 @default.
- W2002168701 hasConceptScore W2002168701C2776580952 @default.
- W2002168701 hasConceptScore W2002168701C2777202666 @default.
- W2002168701 hasConceptScore W2002168701C515207424 @default.
- W2002168701 hasConceptScore W2002168701C55493867 @default.
- W2002168701 hasConceptScore W2002168701C61174792 @default.
- W2002168701 hasConceptScore W2002168701C62231903 @default.
- W2002168701 hasConceptScore W2002168701C86803240 @default.
- W2002168701 hasIssue "7" @default.
- W2002168701 hasLocation W20021687011 @default.
- W2002168701 hasLocation W20021687012 @default.
- W2002168701 hasOpenAccess W2002168701 @default.
- W2002168701 hasPrimaryLocation W20021687011 @default.
- W2002168701 hasRelatedWork W1530697014 @default.
- W2002168701 hasRelatedWork W1930183105 @default.
- W2002168701 hasRelatedWork W1963548841 @default.
- W2002168701 hasRelatedWork W1968086812 @default.
- W2002168701 hasRelatedWork W1995240857 @default.
- W2002168701 hasRelatedWork W2017119362 @default.
- W2002168701 hasRelatedWork W2052537837 @default.
- W2002168701 hasRelatedWork W2162761845 @default.
- W2002168701 hasRelatedWork W2300338905 @default.
- W2002168701 hasRelatedWork W752011107 @default.
- W2002168701 hasVolume "31" @default.
- W2002168701 isParatext "false" @default.
- W2002168701 isRetracted "false" @default.
- W2002168701 magId "2002168701" @default.
- W2002168701 workType "article" @default.