Matches in SemOpenAlex for { <https://semopenalex.org/work/W2002195019> ?p ?o ?g. }
- W2002195019 endingPage "e1004773" @default.
- W2002195019 startingPage "e1004773" @default.
- W2002195019 abstract "Microbial metabolism of plant polysaccharides is an important part of environmental carbon cycling, human nutrition, and industrial processes based on cellulosic bioconversion. Here we demonstrate a broadly applicable method to analyze how microbes catabolize plant polysaccharides that integrates carbohydrate-active enzyme (CAZyme) assays, RNA sequencing (RNA-seq), and anaerobic growth screening. We apply this method to study how the bacterium Clostridium phytofermentans ferments plant biomass components including glucans, mannans, xylans, galactans, pectins, and arabinans. These polysaccharides are fermented with variable efficiencies, and diauxies prioritize metabolism of preferred substrates. Strand-specific RNA-seq reveals how this bacterium responds to polysaccharides by up-regulating specific groups of CAZymes, transporters, and enzymes to metabolize the constituent sugars. Fifty-six up-regulated CAZymes were purified, and their activities show most polysaccharides are degraded by multiple enzymes, often from the same family, but with divergent rates, specificities, and cellular localizations. CAZymes were then tested in combination to identify synergies between enzymes acting on the same substrate with different catalytic mechanisms. We discuss how these results advance our understanding of how microbes degrade and metabolize plant biomass." @default.
- W2002195019 created "2016-06-24" @default.
- W2002195019 creator A5000367016 @default.
- W2002195019 creator A5000482521 @default.
- W2002195019 creator A5013364934 @default.
- W2002195019 creator A5015329541 @default.
- W2002195019 creator A5024898052 @default.
- W2002195019 creator A5054265764 @default.
- W2002195019 creator A5068749773 @default.
- W2002195019 date "2014-11-13" @default.
- W2002195019 modified "2023-09-30" @default.
- W2002195019 title "Functional Diversity of Carbohydrate-Active Enzymes Enabling a Bacterium to Ferment Plant Biomass" @default.
- W2002195019 cites W1484645182 @default.
- W2002195019 cites W1513446746 @default.
- W2002195019 cites W1546730880 @default.
- W2002195019 cites W1819899307 @default.
- W2002195019 cites W1934168465 @default.
- W2002195019 cites W1936378126 @default.
- W2002195019 cites W1963579737 @default.
- W2002195019 cites W1971193134 @default.
- W2002195019 cites W1971324256 @default.
- W2002195019 cites W1973996653 @default.
- W2002195019 cites W1974966154 @default.
- W2002195019 cites W1982575478 @default.
- W2002195019 cites W1982990136 @default.
- W2002195019 cites W1983140978 @default.
- W2002195019 cites W1984818534 @default.
- W2002195019 cites W1987748825 @default.
- W2002195019 cites W1991497480 @default.
- W2002195019 cites W1998783353 @default.
- W2002195019 cites W1999577767 @default.
- W2002195019 cites W2009674410 @default.
- W2002195019 cites W2013699079 @default.
- W2002195019 cites W2025805342 @default.
- W2002195019 cites W2030702588 @default.
- W2002195019 cites W2037885786 @default.
- W2002195019 cites W2038516120 @default.
- W2002195019 cites W2039671341 @default.
- W2002195019 cites W2040048905 @default.
- W2002195019 cites W2054796509 @default.
- W2002195019 cites W2054933711 @default.
- W2002195019 cites W2057671789 @default.
- W2002195019 cites W2059570089 @default.
- W2002195019 cites W2066083913 @default.
- W2002195019 cites W2094201044 @default.
- W2002195019 cites W2094476648 @default.
- W2002195019 cites W2095932050 @default.
- W2002195019 cites W2096488278 @default.
- W2002195019 cites W2098839738 @default.
- W2002195019 cites W2104046482 @default.
- W2002195019 cites W2104852630 @default.
- W2002195019 cites W2104992237 @default.
- W2002195019 cites W2106728647 @default.
- W2002195019 cites W2115981289 @default.
- W2002195019 cites W2116322723 @default.
- W2002195019 cites W2118095887 @default.
- W2002195019 cites W2121107818 @default.
- W2002195019 cites W2122559203 @default.
- W2002195019 cites W2124985265 @default.
- W2002195019 cites W2126728659 @default.
- W2002195019 cites W2133917294 @default.
- W2002195019 cites W2135644384 @default.
- W2002195019 cites W2142521359 @default.
- W2002195019 cites W2144431695 @default.
- W2002195019 cites W2145436266 @default.
- W2002195019 cites W2146098289 @default.
- W2002195019 cites W2146376108 @default.
- W2002195019 cites W2148503872 @default.
- W2002195019 cites W2152239989 @default.
- W2002195019 cites W2152414311 @default.
- W2002195019 cites W2162284435 @default.
- W2002195019 cites W2170430632 @default.
- W2002195019 cites W2329302408 @default.
- W2002195019 cites W2343716889 @default.
- W2002195019 cites W4253164051 @default.
- W2002195019 doi "https://doi.org/10.1371/journal.pgen.1004773" @default.
- W2002195019 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4230839" @default.
- W2002195019 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25393313" @default.
- W2002195019 hasPublicationYear "2014" @default.
- W2002195019 type Work @default.
- W2002195019 sameAs 2002195019 @default.
- W2002195019 citedByCount "79" @default.
- W2002195019 countsByYear W20021950192015 @default.
- W2002195019 countsByYear W20021950192016 @default.
- W2002195019 countsByYear W20021950192017 @default.
- W2002195019 countsByYear W20021950192018 @default.
- W2002195019 countsByYear W20021950192019 @default.
- W2002195019 countsByYear W20021950192020 @default.
- W2002195019 countsByYear W20021950192021 @default.
- W2002195019 countsByYear W20021950192022 @default.
- W2002195019 countsByYear W20021950192023 @default.
- W2002195019 crossrefType "journal-article" @default.
- W2002195019 hasAuthorship W2002195019A5000367016 @default.
- W2002195019 hasAuthorship W2002195019A5000482521 @default.
- W2002195019 hasAuthorship W2002195019A5013364934 @default.
- W2002195019 hasAuthorship W2002195019A5015329541 @default.
- W2002195019 hasAuthorship W2002195019A5024898052 @default.
- W2002195019 hasAuthorship W2002195019A5054265764 @default.