Matches in SemOpenAlex for { <https://semopenalex.org/work/W2002202231> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2002202231 endingPage "350" @default.
- W2002202231 startingPage "350" @default.
- W2002202231 abstract "Introduction: Plant latex is a natural source of biologically active compounds and several hydrolytic enzymes responsible for their diverse health benefits. Recent past has witnessed substantial progress in understanding their supplementary industrial and pharmaceutical utility. Calotropis gigantea is one of the important latex producing plants belonging to asclepediaceae family with wide ethnopharmacological applications and is rich in proteolytic enzymes. Present study investigates hemostatic, milk clotting and blood stain removal potential of C. gigantea latex proteases. Materials and Methods: The protease activity of crude enzyme (CE), obtained by centrifugation followed by ammonium sulphate precipitation and dialysis, was assayed using casein as the substrate. Effect of pH, temperature and specific inhibitors on protease activity was determined. Native PAGE and in gel protease activity of CE was performed. Hemostatic (Fibrinogen polymerization, fibrinogen agarose plate and blood clot lysis assays), milk clotting and blood stain removal efficacies of CE were determined. Results: CE exhibited high caseinolytic activity. Enzyme activity was optimum at 37-50ºC and pH 8.0. Fibrinogen polymerization assay showed concentration dependent increase in turbidity indicating thrombin like activity which was further confirmed by fibrinogen agarose plate assays. Clot lysis assay indicated 92.41% thrombolysis by CE in 90 min. CE also revealed significantly high ratio of milk clotting to protease activity (Milk Clotting Index, MCI = 827.59 ± 1.52). Complete destaining of blood stained fabric was observed when incubated with 1% detergent incorporated with 0.1mg/ml CE. The study highlights and validates the compound application potential of latex cysteine proteases from C. gigantea." @default.
- W2002202231 created "2016-06-24" @default.
- W2002202231 creator A5036895828 @default.
- W2002202231 creator A5062128144 @default.
- W2002202231 date "2014-01-01" @default.
- W2002202231 modified "2023-10-17" @default.
- W2002202231 title "Hemostatic, milk clotting and blood stain removal potential of cysteine proteases from Calotropis gigantea (L.) R. Br. Latex" @default.
- W2002202231 cites W2055253187 @default.
- W2002202231 cites W2199947764 @default.
- W2002202231 cites W2313056181 @default.
- W2002202231 cites W1004622453 @default.
- W2002202231 doi "https://doi.org/10.4103/0973-1296.133294" @default.
- W2002202231 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4078356" @default.
- W2002202231 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24991114" @default.
- W2002202231 hasPublicationYear "2014" @default.
- W2002202231 type Work @default.
- W2002202231 sameAs 2002202231 @default.
- W2002202231 citedByCount "13" @default.
- W2002202231 countsByYear W20022022312015 @default.
- W2002202231 countsByYear W20022022312016 @default.
- W2002202231 countsByYear W20022022312017 @default.
- W2002202231 countsByYear W20022022312018 @default.
- W2002202231 countsByYear W20022022312019 @default.
- W2002202231 countsByYear W20022022312020 @default.
- W2002202231 countsByYear W20022022312021 @default.
- W2002202231 countsByYear W20022022312022 @default.
- W2002202231 countsByYear W20022022312023 @default.
- W2002202231 crossrefType "journal-article" @default.
- W2002202231 hasAuthorship W2002202231A5036895828 @default.
- W2002202231 hasAuthorship W2002202231A5062128144 @default.
- W2002202231 hasBestOaLocation W20022022312 @default.
- W2002202231 hasConcept C118552586 @default.
- W2002202231 hasConcept C181199279 @default.
- W2002202231 hasConcept C182220744 @default.
- W2002202231 hasConcept C185592680 @default.
- W2002202231 hasConcept C2776714187 @default.
- W2002202231 hasConcept C2778382381 @default.
- W2002202231 hasConcept C2778476535 @default.
- W2002202231 hasConcept C3019223276 @default.
- W2002202231 hasConcept C43617362 @default.
- W2002202231 hasConcept C55493867 @default.
- W2002202231 hasConcept C71924100 @default.
- W2002202231 hasConcept C77171857 @default.
- W2002202231 hasConceptScore W2002202231C118552586 @default.
- W2002202231 hasConceptScore W2002202231C181199279 @default.
- W2002202231 hasConceptScore W2002202231C182220744 @default.
- W2002202231 hasConceptScore W2002202231C185592680 @default.
- W2002202231 hasConceptScore W2002202231C2776714187 @default.
- W2002202231 hasConceptScore W2002202231C2778382381 @default.
- W2002202231 hasConceptScore W2002202231C2778476535 @default.
- W2002202231 hasConceptScore W2002202231C3019223276 @default.
- W2002202231 hasConceptScore W2002202231C43617362 @default.
- W2002202231 hasConceptScore W2002202231C55493867 @default.
- W2002202231 hasConceptScore W2002202231C71924100 @default.
- W2002202231 hasConceptScore W2002202231C77171857 @default.
- W2002202231 hasIssue "38" @default.
- W2002202231 hasLocation W20022022311 @default.
- W2002202231 hasLocation W20022022312 @default.
- W2002202231 hasLocation W20022022313 @default.
- W2002202231 hasLocation W20022022314 @default.
- W2002202231 hasOpenAccess W2002202231 @default.
- W2002202231 hasPrimaryLocation W20022022311 @default.
- W2002202231 hasRelatedWork W124183450 @default.
- W2002202231 hasRelatedWork W1596676072 @default.
- W2002202231 hasRelatedWork W2068975409 @default.
- W2002202231 hasRelatedWork W2109018839 @default.
- W2002202231 hasRelatedWork W2124956779 @default.
- W2002202231 hasRelatedWork W2153970031 @default.
- W2002202231 hasRelatedWork W2159000061 @default.
- W2002202231 hasRelatedWork W2182122486 @default.
- W2002202231 hasRelatedWork W3195890830 @default.
- W2002202231 hasRelatedWork W3207552102 @default.
- W2002202231 hasVolume "10" @default.
- W2002202231 isParatext "false" @default.
- W2002202231 isRetracted "false" @default.
- W2002202231 magId "2002202231" @default.
- W2002202231 workType "article" @default.