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• W2002204620 startingPage "153" @default.
• W2002204620 abstract "A human autoimmune serum to nucleolus organizer regions (NORs) has been used to localize these structures at the light microscopic level in carp and trout tissue culture cells. In interphase cells, the immunofluorescence pattern indicates that the NORs autoantigens are contained exclusively within the nucleolus of carp epithelial (EPC) and trout gonad (RTG) cells. This fluorescence is punctuate rather than uniform, and presumably represents transcriptional complexes of ribosomal DNA. During mitosis, the autoantigens are detected by immunofluorescence microscopy at the chromosomal nucleolus organizer regions of condensed chromosomes, indicating that a considerable quantity of the molecule(s) remains bound to the ribosomal RNA genes. The major nucleolus autoantigen, denned in mammals as the upstream ribosomal binding factor (UBF), has been identified on immunoblots as a 90 kDa protein in extracts from fish cell lines and tissues. Thus, NORs appear to function as nucleation centers for ribosomal RNA together with a complex set of well-conserved protein factors, such as UBF. Our results suggest evolutionary conservation from fish to mammals with respect to ribosomal RNA biosynthesis driven by RNA polymerase I." @default.
• W2002204620 created "2016-06-24" @default.
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• W2002204620 date "1994-01-01" @default.
• W2002204620 modified "2023-09-24" @default.
• W2002204620 title "Immunodetection of the Ribosomal Transcription Factor UBF at the Nucleolus Organizer Regions of Fish Cells." @default.
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• W2002204620 doi "https://doi.org/10.1247/csf.19.153" @default.
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