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• W2002237669 startingPage "419" @default.
• W2002237669 abstract "The enzymatic incorporation of a series of emissive pyrimidine analogues into RNA oligonucleotides is explored. T7 RNA polymerase is challenged with accepting three non-natural, yet related, triphosphates as substrates and incorporating them into diverse RNA transcripts. The three ribonucleoside triphosphates differ only in the modification of their uracil nucleus and include a thieno[3,2-d]pyrimidine nucleoside, a thieno[3,4-d]pyrimidine derivative, and a uridine containing a thiophene ring conjugated at its 5-position. All thiophene-containing uridine triphosphates (UTPs) get incorporated into RNA oligonucleotides at positions that are remote to the promoter, although the yields of the transcripts vary compared with the transcript obtained with only native triphosphates. Among the three derivatives, the 5-modified UTP is found to be the most polymerase-friendly and is well accommodated by T7 RNA polymerase. Although the fused thiophene analogues cannot be incorporated next to the promoter region, the 5-modified non-natural UTP gets incorporated near the promoter (albeit in relatively low yields) and even in multiple copies. Labeling experiments shed light on the mediocre incorporation of the fused analogues, suggesting the enzyme frequently pauses at the incorporation position. When incorporation does take place, the enzyme fails to elongate the modified oligonucleotide and yields aborted transcripts. Taken together, these results highlight the versatility and robustness, as well as the scope and limitation, of T7 RNA polymerase in accepting and incorporating reporter nucleotides into modified RNA transcripts." @default.
• W2002237669 created "2016-06-24" @default.
• W2002237669 creator A5014322047 @default.
• W2002237669 creator A5066827195 @default.
• W2002237669 date "2009-02-24" @default.
• W2002237669 modified "2023-10-12" @default.
• W2002237669 title "Enzymatic Incorporation of Emissive Pyrimidine Ribonucleotides" @default.
• W2002237669 cites W1498439983 @default.
• W2002237669 cites W1510602108 @default.
• W2002237669 cites W1568303364 @default.
• W2002237669 cites W1897074664 @default.
• W2002237669 cites W1964102227 @default.
• W2002237669 cites W1964455506 @default.
• W2002237669 cites W1966139782 @default.
• W2002237669 cites W1966524679 @default.
• W2002237669 cites W1967121834 @default.
• W2002237669 cites W1968000756 @default.
• W2002237669 cites W1972980824 @default.
• W2002237669 cites W1974955901 @default.
• W2002237669 cites W1976818324 @default.
• W2002237669 cites W1977619408 @default.
• W2002237669 cites W1978298790 @default.
• W2002237669 cites W1982328110 @default.
• W2002237669 cites W1985790087 @default.
• W2002237669 cites W1986495208 @default.
• W2002237669 cites W1986588909 @default.
• W2002237669 cites W1988496377 @default.
• W2002237669 cites W1989454102 @default.
• W2002237669 cites W1989920813 @default.
• W2002237669 cites W1991015650 @default.
• W2002237669 cites W1994016687 @default.
• W2002237669 cites W1994693974 @default.
• W2002237669 cites W2000372394 @default.
• W2002237669 cites W2005942279 @default.
• W2002237669 cites W2007223845 @default.
• W2002237669 cites W2007899774 @default.
• W2002237669 cites W2010454871 @default.
• W2002237669 cites W2010640188 @default.
• W2002237669 cites W2010767982 @default.
• W2002237669 cites W2014038908 @default.
• W2002237669 cites W2014946489 @default.
• W2002237669 cites W2015189505 @default.
• W2002237669 cites W2016114120 @default.
• W2002237669 cites W2016147779 @default.
• W2002237669 cites W2019368503 @default.
• W2002237669 cites W2022252180 @default.
• W2002237669 cites W2022890528 @default.
• W2002237669 cites W2025538879 @default.
• W2002237669 cites W2025670433 @default.
• W2002237669 cites W2029443955 @default.
• W2002237669 cites W2035311056 @default.
• W2002237669 cites W2036332544 @default.
• W2002237669 cites W2037940313 @default.
• W2002237669 cites W2038522230 @default.
• W2002237669 cites W2039533427 @default.
• W2002237669 cites W2041520261 @default.
• W2002237669 cites W2041889200 @default.
• W2002237669 cites W2048427760 @default.
• W2002237669 cites W2048791248 @default.
• W2002237669 cites W2049105226 @default.
• W2002237669 cites W2051444527 @default.
• W2002237669 cites W2053771753 @default.
• W2002237669 cites W2059214166 @default.
• W2002237669 cites W2059668879 @default.
• W2002237669 cites W2060840840 @default.
• W2002237669 cites W2062544204 @default.
• W2002237669 cites W2063532087 @default.
• W2002237669 cites W2065164352 @default.
• W2002237669 cites W2067655705 @default.
• W2002237669 cites W2070339052 @default.
• W2002237669 cites W2072263795 @default.
• W2002237669 cites W2077275642 @default.
• W2002237669 cites W2079619138 @default.
• W2002237669 cites W2079650265 @default.
• W2002237669 cites W2082592915 @default.
• W2002237669 cites W2085272777 @default.
• W2002237669 cites W2085391689 @default.
• W2002237669 cites W2085743163 @default.
• W2002237669 cites W2089176153 @default.
• W2002237669 cites W2089938533 @default.
• W2002237669 cites W2091438758 @default.
• W2002237669 cites W2093999832 @default.
• W2002237669 cites W2095613131 @default.
• W2002237669 cites W2097617128 @default.
• W2002237669 cites W2106452529 @default.
• W2002237669 cites W2106836466 @default.
• W2002237669 cites W2109936378 @default.
• W2002237669 cites W2111261502 @default.
• W2002237669 cites W2114156541 @default.
• W2002237669 cites W2115120038 @default.
• W2002237669 cites W2122282339 @default.
• W2002237669 cites W2123206012 @default.
• W2002237669 cites W2123481481 @default.
• W2002237669 cites W2129995310 @default.
• W2002237669 cites W2134508771 @default.
• W2002237669 cites W2134952259 @default.
• W2002237669 cites W2135817740 @default.
• W2002237669 cites W2145988980 @default.

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