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- W2002244856 abstract "In the detachment process of human keratinocyte cells with trypsin, morphological changes of individual cells were observed to estimate the cellular property. The kinetic parameters, αi/k and δi/k, were proposed as indices concerning the adhesive force of each cell and density of adhesive strength, respectively, and these parameters were determined from the time profiles of cell shrinkage during the trypsinization. The value of αi/k decreased with detachment process time, tT, and ultimately entered into a region of αi/k=0–4.0 mm s at tT=720 s. The profile of cell detachment could be described in fair agreement with experimental data, by considering that the cells with less than αi/k=4.0 mm s were detached ones from the surface. In subsequent cultures of the cells detached during trypsinization, it was found that the cells included in a fraction with small δi/k values exhibited the deteriorated growth potential. It was thus demonstrated that the proposed parameters could be useful measures to predict the adhesive and proliferative abilities of the anchorage-dependent cells." @default.
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- W2002244856 date "2004-01-01" @default.
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- W2002244856 title "Assessment of cell detachment and growth potential of human keratinocyte based on observed changes in individual cell area during trypsinization" @default.
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- W2002244856 doi "https://doi.org/10.1016/s1369-703x(03)00124-4" @default.
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