Matches in SemOpenAlex for { <https://semopenalex.org/work/W2002246211> ?p ?o ?g. }
- W2002246211 endingPage "333" @default.
- W2002246211 startingPage "317" @default.
- W2002246211 abstract "We had shown previously that DNA polymerase beta (beta-pol) null mouse fibroblasts, deficient in base excision repair (BER), are hypersensitive to monofunctional methylating agents but not to hydrogen peroxide (H2O2). This is surprising because beta-pol is thought to be involved in BER of oxidative as well as methylated DNA damage. We confirm these findings here in early-passage cells. However, with time in culture, beta-pol null cells become hypersensitive to H2O2 and other reactive oxygen species-generating agents. Analysis of in vitro BER reveals a strong deficiency in single-nucleotide BER of 8-oxoguanine (8-oxoG) by both early- and late-passage beta-pol null cell extracts. Therefore, in early-passage wild-type and beta-pol null cells, the capacity for single-nucleotide BER of 8-oxoG does not correlate with cellular sensitivity to H2O2. Expression of beta-pol protein in the late-passage null cells almost completely reverses the H2O2-hypersensitivity phenotype. Methoxyamine (MX) treatment sensitizes late-passage wild-type cells to H2O2 as expected for beta-pol-mediated single-nucleotide BER; however in beta-pol null cells, MX has no effect. The data indicate a role(s) of beta-pol-dependent repair in protection against the cytotoxicity of oxidative DNA damage in wild-type cells." @default.
- W2002246211 created "2016-06-24" @default.
- W2002246211 creator A5010157219 @default.
- W2002246211 creator A5027876809 @default.
- W2002246211 creator A5045523035 @default.
- W2002246211 creator A5066236513 @default.
- W2002246211 creator A5073304204 @default.
- W2002246211 date "2002-04-01" @default.
- W2002246211 modified "2023-09-23" @default.
- W2002246211 title "Involvement of DNA polymerase β in protection against the cytotoxicity of oxidative DNA damage" @default.
- W2002246211 cites W1584549675 @default.
- W2002246211 cites W1970631404 @default.
- W2002246211 cites W1971040146 @default.
- W2002246211 cites W1971602039 @default.
- W2002246211 cites W1973194906 @default.
- W2002246211 cites W1974437539 @default.
- W2002246211 cites W1974585689 @default.
- W2002246211 cites W1974762401 @default.
- W2002246211 cites W1983795887 @default.
- W2002246211 cites W1983825041 @default.
- W2002246211 cites W1983863777 @default.
- W2002246211 cites W1984656756 @default.
- W2002246211 cites W1989168350 @default.
- W2002246211 cites W1995484312 @default.
- W2002246211 cites W2000479074 @default.
- W2002246211 cites W2007064404 @default.
- W2002246211 cites W2007998684 @default.
- W2002246211 cites W2012291005 @default.
- W2002246211 cites W2014819546 @default.
- W2002246211 cites W2019777050 @default.
- W2002246211 cites W2020088400 @default.
- W2002246211 cites W2023256344 @default.
- W2002246211 cites W2033192724 @default.
- W2002246211 cites W2035688945 @default.
- W2002246211 cites W2041045544 @default.
- W2002246211 cites W2042699531 @default.
- W2002246211 cites W2048527989 @default.
- W2002246211 cites W2052350481 @default.
- W2002246211 cites W2059123383 @default.
- W2002246211 cites W2061270600 @default.
- W2002246211 cites W2064477710 @default.
- W2002246211 cites W2070572828 @default.
- W2002246211 cites W2072695826 @default.
- W2002246211 cites W2081048715 @default.
- W2002246211 cites W2085312575 @default.
- W2002246211 cites W2087342144 @default.
- W2002246211 cites W2087917559 @default.
- W2002246211 cites W2105640380 @default.
- W2002246211 cites W2106751562 @default.
- W2002246211 cites W2113014296 @default.
- W2002246211 cites W2114024672 @default.
- W2002246211 cites W2131774991 @default.
- W2002246211 cites W2134316981 @default.
- W2002246211 cites W2134831636 @default.
- W2002246211 cites W2149922717 @default.
- W2002246211 cites W2151444462 @default.
- W2002246211 cites W2152116092 @default.
- W2002246211 cites W2154690470 @default.
- W2002246211 cites W2163663923 @default.
- W2002246211 cites W2165432409 @default.
- W2002246211 cites W2168882862 @default.
- W2002246211 cites W2216471839 @default.
- W2002246211 cites W2327068644 @default.
- W2002246211 cites W2398980959 @default.
- W2002246211 cites W4246036060 @default.
- W2002246211 cites W4252805882 @default.
- W2002246211 doi "https://doi.org/10.1016/s1568-7864(02)00008-3" @default.
- W2002246211 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12509250" @default.
- W2002246211 hasPublicationYear "2002" @default.
- W2002246211 type Work @default.
- W2002246211 sameAs 2002246211 @default.
- W2002246211 citedByCount "78" @default.
- W2002246211 countsByYear W20022462112012 @default.
- W2002246211 countsByYear W20022462112013 @default.
- W2002246211 countsByYear W20022462112014 @default.
- W2002246211 countsByYear W20022462112015 @default.
- W2002246211 countsByYear W20022462112016 @default.
- W2002246211 countsByYear W20022462112017 @default.
- W2002246211 countsByYear W20022462112018 @default.
- W2002246211 countsByYear W20022462112019 @default.
- W2002246211 countsByYear W20022462112021 @default.
- W2002246211 countsByYear W20022462112022 @default.
- W2002246211 countsByYear W20022462112023 @default.
- W2002246211 crossrefType "journal-article" @default.
- W2002246211 hasAuthorship W2002246211A5010157219 @default.
- W2002246211 hasAuthorship W2002246211A5027876809 @default.
- W2002246211 hasAuthorship W2002246211A5045523035 @default.
- W2002246211 hasAuthorship W2002246211A5066236513 @default.
- W2002246211 hasAuthorship W2002246211A5073304204 @default.
- W2002246211 hasConcept C104317684 @default.
- W2002246211 hasConcept C121853132 @default.
- W2002246211 hasConcept C134935766 @default.
- W2002246211 hasConcept C143425029 @default.
- W2002246211 hasConcept C153911025 @default.
- W2002246211 hasConcept C187206112 @default.
- W2002246211 hasConcept C199360897 @default.
- W2002246211 hasConcept C2756471 @default.
- W2002246211 hasConcept C2776174256 @default.