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- W2002309156 abstract "The biotransformation of poorly water soluble aromatic dinitriles is of industrial and scientific interest. Although processes do exist for the transformation of water soluble nitriles, such as acrylonitrile, no description of a process suitable for the large scale biotransformation of poorly water soluble nitriles appears in the literature. In this work we illustrate a systematic design procedure for optimising the production of 3-cyanobenzamide from 1,3-dicyanobenzene (1,3-DCB). The regio-selective nitrile hydratase (NHase) of the well characterised Rhodococcus R312 strain was initially selected as catalyst. Isolation of the NHase at process scale however was not feasible due to the rigid cell wall of the bacteria and the poor stability of the isolated enzyme. The whole cell form of the biocatalyst was thus used even though the activity of the associated amidase could overmetabolise the amide product into the corresponding acid. To overcome productivity limitations imposed by the characteristically low ..." @default.
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- W2002309156 date "2001-01-01" @default.
- W2002309156 modified "2023-09-23" @default.
- W2002309156 title "Process Selection and Characterisation for the Biocatalytic Hydration of Poorly Water Soluble Aromatic Dinitriles" @default.
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- W2002309156 doi "https://doi.org/10.3109/10242420109003641" @default.
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