FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2002338593> ?p ?o ?g. }

• W2002338593 endingPage "e83563" @default.
• W2002338593 startingPage "e83563" @default.
• W2002338593 abstract "We previously reported that matrix metalloproteinase (MMP)-3 accelerates wound healing following dental pulp injury. In addition, we reported that a proinflammatory cytokine mixture (tumor necrosis factor-α, interleukin (IL)-1β and interferon-γ) induced MMP-3 activity in odontoblast-like cells derived from mouse embryonic stem (ES) cells, suggesting that MMP-3 plays a potential unique physiological role in wound healing and regeneration of dental pulp in odontoblast-like cells. In this study, we tested the hypothesis that upregulation of MMP-3 activity by IL-1β promotes proliferation and apoptosis of purified odontoblast-like cells derived from induced pluripotent stem (iPS) and ES cells. Each odontoblast-like cell was isolated and incubated with different concentrations of IL-1β. MMP-3 mRNA and protein expression were assessed using RT-PCR and western blotting, respectively. MMP-3 activity was measured using immunoprecipitation and a fluorescence substrate. Cell proliferation and apoptosis were determined using ELISA for BrdU and DNA fragmentation, respectively. siRNA was used to reduce MMP-3 transcripts in these cells. Treatment with IL-1β increased MMP-3 mRNA and protein levels, and MMP-3 activity in odontoblast-like cells. Cell proliferation was found to markedly increase with no changes in apoptosis. Endogenous tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 were constitutively expressed during all experiments. The exocytosis inhibitor, Exo1, potently suppressed the appearance of MMP-3 in the conditioned medium. Treatment with siRNA against MMP-3 suppressed an IL-1β-induced increase in MMP-3 expression and activity, and also suppressed cell proliferation, but unexpectedly increased apoptosis in these cells (P<0.05). Exogenous MMP-3 was found to induce cell proliferation in odontoblast-like cells derived from iPS cells and ES cells. This siRNA-mediated increase in apoptosis could be reversed with exogenous MMP-3 stimulation (P<0.05). Taken together, IL-1β induced MMP-3-regulated cell proliferation and suppressed apoptosis in odontoblast-like cells derived from iPS and ES cells." @default.
• W2002338593 created "2016-06-24" @default.
• W2002338593 creator A5011585554 @default.
• W2002338593 creator A5044988806 @default.
• W2002338593 creator A5051499809 @default.
• W2002338593 creator A5062640038 @default.
• W2002338593 creator A5081862693 @default.
• W2002338593 creator A5087699141 @default.
• W2002338593 creator A5090578339 @default.
• W2002338593 creator A5091589039 @default.
• W2002338593 date "2013-12-16" @default.
• W2002338593 modified "2023-10-14" @default.
• W2002338593 title "Matrix Metalloproteinase-3 in Odontoblastic Cells Derived from Ips Cells: Unique Proliferation Response as Odontoblastic Cells Derived from ES Cells" @default.
• W2002338593 cites W1556396967 @default.
• W2002338593 cites W1800142641 @default.
• W2002338593 cites W1964935717 @default.
• W2002338593 cites W1966180339 @default.
• W2002338593 cites W1970293798 @default.
• W2002338593 cites W1972957895 @default.
• W2002338593 cites W1974898266 @default.
• W2002338593 cites W1985310530 @default.
• W2002338593 cites W1986892166 @default.
• W2002338593 cites W2003902924 @default.
• W2002338593 cites W2009097861 @default.
• W2002338593 cites W2018252879 @default.
• W2002338593 cites W2022211106 @default.
• W2002338593 cites W2032586069 @default.
• W2002338593 cites W2035223569 @default.
• W2002338593 cites W2036559032 @default.
• W2002338593 cites W2039063705 @default.
• W2002338593 cites W2043462986 @default.
• W2002338593 cites W2053069753 @default.
• W2002338593 cites W2053700159 @default.
• W2002338593 cites W2064658722 @default.
• W2002338593 cites W2069765642 @default.
• W2002338593 cites W2072540407 @default.
• W2002338593 cites W2072686928 @default.
• W2002338593 cites W2073397502 @default.
• W2002338593 cites W2076381272 @default.
• W2002338593 cites W2080644261 @default.
• W2002338593 cites W2083573042 @default.
• W2002338593 cites W2084150311 @default.
• W2002338593 cites W2091663966 @default.
• W2002338593 cites W2093756962 @default.
• W2002338593 cites W2098498195 @default.
• W2002338593 cites W2104526708 @default.
• W2002338593 cites W2109658199 @default.
• W2002338593 cites W2121153471 @default.
• W2002338593 cites W2124501705 @default.
• W2002338593 cites W2125987139 @default.
• W2002338593 cites W2129729644 @default.
• W2002338593 cites W2130626603 @default.
• W2002338593 cites W2131970139 @default.
• W2002338593 cites W2138977668 @default.
• W2002338593 cites W2146331718 @default.
• W2002338593 cites W2154401382 @default.
• W2002338593 cites W2166091458 @default.
• W2002338593 cites W4250908540 @default.
• W2002338593 doi "https://doi.org/10.1371/journal.pone.0083563" @default.
• W2002338593 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3865184" @default.
• W2002338593 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24358294" @default.
• W2002338593 hasPublicationYear "2013" @default.
• W2002338593 type Work @default.
• W2002338593 sameAs 2002338593 @default.
• W2002338593 citedByCount "24" @default.
• W2002338593 countsByYear W20023385932014 @default.
• W2002338593 countsByYear W20023385932015 @default.
• W2002338593 countsByYear W20023385932016 @default.
• W2002338593 countsByYear W20023385932017 @default.
• W2002338593 countsByYear W20023385932018 @default.
• W2002338593 countsByYear W20023385932019 @default.
• W2002338593 countsByYear W20023385932021 @default.
• W2002338593 crossrefType "journal-article" @default.
• W2002338593 hasAuthorship W2002338593A5011585554 @default.
• W2002338593 hasAuthorship W2002338593A5044988806 @default.
• W2002338593 hasAuthorship W2002338593A5051499809 @default.
• W2002338593 hasAuthorship W2002338593A5062640038 @default.
• W2002338593 hasAuthorship W2002338593A5081862693 @default.
• W2002338593 hasAuthorship W2002338593A5087699141 @default.
• W2002338593 hasAuthorship W2002338593A5090578339 @default.
• W2002338593 hasAuthorship W2002338593A5091589039 @default.
• W2002338593 hasBestOaLocation W20023385931 @default.
• W2002338593 hasConcept C109523444 @default.
• W2002338593 hasConcept C142724271 @default.
• W2002338593 hasConcept C153911025 @default.
• W2002338593 hasConcept C185592680 @default.
• W2002338593 hasConcept C188442228 @default.
• W2002338593 hasConcept C190283241 @default.
• W2002338593 hasConcept C203014093 @default.
• W2002338593 hasConcept C2520015 @default.
• W2002338593 hasConcept C2777400515 @default.
• W2002338593 hasConcept C2780269544 @default.
• W2002338593 hasConcept C28328180 @default.
• W2002338593 hasConcept C55493867 @default.
• W2002338593 hasConcept C62112901 @default.
• W2002338593 hasConcept C71924100 @default.
• W2002338593 hasConcept C86803240 @default.
• W2002338593 hasConcept C95444343 @default.

• next