FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2002387734> ?p ?o ?g. }

• W2002387734 endingPage "108" @default.
• W2002387734 startingPage "97" @default.
• W2002387734 abstract "In the maize endosperm, the Opaque2 (O2) basic leucine zipper transcriptional activator regulates the expression of a subset of the zein seed storage protein gene family. Immunodetection of wild-type or mutant O2 polypeptides fractionated by SDS-PAGE resolved a closely spaced doublet migrating in the 68- to 72-kD range, whereas by using isoelectric focusing, seven to nine isoforms were detected for each allele. Phosphatase treatment simplified the protein patterns to a single band corresponding to the nonphosphorylated component. In vivo and in vitro labeling confirmed that O2 can be phosphorylated. In protein gel blots probed with DNA, only the nonphosphorylated and hypophosphorylated O2 polypeptides were able to bind an oligonucleotide containing the O2 binding sequence. Upon in situ dephosphorylation of the focused isoforms by phosphatase treatment of the isoelectric focusing filter, the hyperphosphorylated forms acquired DNA binding activity. The ratio among the various isoforms remained constant throughout the developmental stages of endosperm growth but changed from daytime to nighttime, with a significant increase of the hyperphosphorylated forms during the night period. These results indicate that O2 exists in vivo as a pool of differently phosphorylated polypeptides and demonstrate that O2 DNA binding activity is modulated by a phosphorylation/dephosphorylation mechanism that appears to be influenced by environmental conditions." @default.
• W2002387734 created "2016-06-24" @default.
• W2002387734 creator A5023256975 @default.
• W2002387734 creator A5025684816 @default.
• W2002387734 creator A5030198404 @default.
• W2002387734 creator A5034836248 @default.
• W2002387734 creator A5035257172 @default.
• W2002387734 creator A5042603393 @default.
• W2002387734 creator A5075206104 @default.
• W2002387734 creator A5075937196 @default.
• W2002387734 date "1997-01-01" @default.
• W2002387734 modified "2023-09-28" @default.
• W2002387734 title "Phosphorylation of Opaque2 changes diurnally and impacts its DNA binding activity." @default.
• W2002387734 cites W1533579770 @default.
• W2002387734 cites W1873199501 @default.
• W2002387734 cites W1969369191 @default.
• W2002387734 cites W1970227360 @default.
• W2002387734 cites W1977302628 @default.
• W2002387734 cites W2000217273 @default.
• W2002387734 cites W2005131012 @default.
• W2002387734 cites W2005673211 @default.
• W2002387734 cites W2006579426 @default.
• W2002387734 cites W2015359212 @default.
• W2002387734 cites W2018808488 @default.
• W2002387734 cites W2019054686 @default.
• W2002387734 cites W2022796740 @default.
• W2002387734 cites W2048481226 @default.
• W2002387734 cites W2057350299 @default.
• W2002387734 cites W2060475895 @default.
• W2002387734 cites W2062075221 @default.
• W2002387734 cites W2082797919 @default.
• W2002387734 cites W2084548353 @default.
• W2002387734 cites W2091285777 @default.
• W2002387734 cites W2091856362 @default.
• W2002387734 cites W2092402636 @default.
• W2002387734 cites W2098141644 @default.
• W2002387734 cites W2104600301 @default.
• W2002387734 cites W2107332488 @default.
• W2002387734 cites W2108090113 @default.
• W2002387734 cites W2109527872 @default.
• W2002387734 cites W2115168068 @default.
• W2002387734 cites W2124579590 @default.
• W2002387734 cites W2129364746 @default.
• W2002387734 cites W2130286797 @default.
• W2002387734 cites W2130343789 @default.
• W2002387734 cites W2156635145 @default.
• W2002387734 cites W2156899368 @default.
• W2002387734 cites W2158808309 @default.
• W2002387734 cites W2166889494 @default.
• W2002387734 cites W2321009361 @default.
• W2002387734 cites W45974969 @default.
• W2002387734 doi "https://doi.org/10.1105/tpc.9.1.97" @default.
• W2002387734 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/156904" @default.
• W2002387734 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9014367" @default.
• W2002387734 hasPublicationYear "1997" @default.
• W2002387734 type Work @default.
• W2002387734 sameAs 2002387734 @default.
• W2002387734 citedByCount "83" @default.
• W2002387734 countsByYear W20023877342012 @default.
• W2002387734 countsByYear W20023877342013 @default.
• W2002387734 countsByYear W20023877342014 @default.
• W2002387734 countsByYear W20023877342015 @default.
• W2002387734 countsByYear W20023877342018 @default.
• W2002387734 countsByYear W20023877342019 @default.
• W2002387734 countsByYear W20023877342020 @default.
• W2002387734 countsByYear W20023877342022 @default.
• W2002387734 countsByYear W20023877342023 @default.
• W2002387734 crossrefType "journal-article" @default.
• W2002387734 hasAuthorship W2002387734A5023256975 @default.
• W2002387734 hasAuthorship W2002387734A5025684816 @default.
• W2002387734 hasAuthorship W2002387734A5030198404 @default.
• W2002387734 hasAuthorship W2002387734A5034836248 @default.
• W2002387734 hasAuthorship W2002387734A5035257172 @default.
• W2002387734 hasAuthorship W2002387734A5042603393 @default.
• W2002387734 hasAuthorship W2002387734A5075206104 @default.
• W2002387734 hasAuthorship W2002387734A5075937196 @default.
• W2002387734 hasBestOaLocation W20023877341 @default.
• W2002387734 hasConcept C104317684 @default.
• W2002387734 hasConcept C11960822 @default.
• W2002387734 hasConcept C143065580 @default.
• W2002387734 hasConcept C153911025 @default.
• W2002387734 hasConcept C156860981 @default.
• W2002387734 hasConcept C178666793 @default.
• W2002387734 hasConcept C181199279 @default.
• W2002387734 hasConcept C181912034 @default.
• W2002387734 hasConcept C2779933727 @default.
• W2002387734 hasConcept C50476208 @default.
• W2002387734 hasConcept C53345823 @default.
• W2002387734 hasConcept C552990157 @default.
• W2002387734 hasConcept C55493867 @default.
• W2002387734 hasConcept C86339819 @default.
• W2002387734 hasConcept C86803240 @default.
• W2002387734 hasConceptScore W2002387734C104317684 @default.
• W2002387734 hasConceptScore W2002387734C11960822 @default.
• W2002387734 hasConceptScore W2002387734C143065580 @default.
• W2002387734 hasConceptScore W2002387734C153911025 @default.
• W2002387734 hasConceptScore W2002387734C156860981 @default.
• W2002387734 hasConceptScore W2002387734C178666793 @default.

• next