Matches in SemOpenAlex for { <https://semopenalex.org/work/W2002497834> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2002497834 endingPage "180" @default.
- W2002497834 startingPage "174" @default.
- W2002497834 abstract "An oxidized derivative of linoleic acid, 13-hydroxyoctadecadienoic acid (13-HODE), is dehydrogenated by an NAD+ dependent dehydrogenase present in rat colon mucosa. The product of the reaction is the 2,4-dienone, 13-oxooctadecadienoic acid. Enzyme activity was determined by HPLC analysis of incubation mixtures as well as by measuring the increase in absorbance at 285 nm, which represents formation of the 2,4-dienone chromophore. Characteristics of the reaction with respect to protein concentration, time of incubation and substrate dependence were investigated. Several inhibitors of known dehydrogenases had no effect on the 13-HODE dehydrogenase. These include, ethanol, indomethacin, 6-methyl-17-hydroxyprogesterone acetate, 4-(diethylamino)-benzaldehyde, and aspirin. The enzyme was mildly inhibited by pyrazole, 4-methylpyrazole and ibuprofen. Disulfiram was found to be a potent inhibitor of enzyme activity with an IC50 of 200 microM. Inhibitor specificity, and other characteristics of the reaction suggest the enzyme is neither alcohol dehydrogenase, diol dehydrogenase, nor a prostaglandin dehydrogenase. It is possible this enzyme plays an important role in the response of the colonic mucosa to the mitogenic effect of oxidized fatty acids." @default.
- W2002497834 created "2016-06-24" @default.
- W2002497834 creator A5001342347 @default.
- W2002497834 creator A5043268668 @default.
- W2002497834 creator A5068251176 @default.
- W2002497834 creator A5088432120 @default.
- W2002497834 date "1991-01-01" @default.
- W2002497834 modified "2023-10-01" @default.
- W2002497834 title "Metabolism of oxidized linoleic acid: characterization of 13-hydroxyoctadecadienoic acid dehydrogenase activity from rat colonic tissue" @default.
- W2002497834 cites W107907750 @default.
- W2002497834 cites W123822435 @default.
- W2002497834 cites W1534343559 @default.
- W2002497834 cites W1558285415 @default.
- W2002497834 cites W1561395636 @default.
- W2002497834 cites W1589895100 @default.
- W2002497834 cites W1947800358 @default.
- W2002497834 cites W1967587270 @default.
- W2002497834 cites W1967818422 @default.
- W2002497834 cites W1972096233 @default.
- W2002497834 cites W1992006648 @default.
- W2002497834 cites W2012716496 @default.
- W2002497834 cites W2026797578 @default.
- W2002497834 cites W2027684589 @default.
- W2002497834 cites W2046630732 @default.
- W2002497834 cites W2047380147 @default.
- W2002497834 cites W2062207244 @default.
- W2002497834 cites W2064162536 @default.
- W2002497834 cites W2064993452 @default.
- W2002497834 cites W2080822276 @default.
- W2002497834 cites W2108682052 @default.
- W2002497834 cites W2159597833 @default.
- W2002497834 cites W2171888981 @default.
- W2002497834 cites W2183070728 @default.
- W2002497834 cites W4293247451 @default.
- W2002497834 doi "https://doi.org/10.1016/0005-2760(91)90023-b" @default.
- W2002497834 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1998735" @default.
- W2002497834 hasPublicationYear "1991" @default.
- W2002497834 type Work @default.
- W2002497834 sameAs 2002497834 @default.
- W2002497834 citedByCount "50" @default.
- W2002497834 countsByYear W20024978342012 @default.
- W2002497834 countsByYear W20024978342015 @default.
- W2002497834 countsByYear W20024978342016 @default.
- W2002497834 countsByYear W20024978342017 @default.
- W2002497834 countsByYear W20024978342018 @default.
- W2002497834 countsByYear W20024978342020 @default.
- W2002497834 countsByYear W20024978342022 @default.
- W2002497834 countsByYear W20024978342023 @default.
- W2002497834 crossrefType "journal-article" @default.
- W2002497834 hasAuthorship W2002497834A5001342347 @default.
- W2002497834 hasAuthorship W2002497834A5043268668 @default.
- W2002497834 hasAuthorship W2002497834A5068251176 @default.
- W2002497834 hasAuthorship W2002497834A5088432120 @default.
- W2002497834 hasConcept C181199279 @default.
- W2002497834 hasConcept C185592680 @default.
- W2002497834 hasConcept C2776317432 @default.
- W2002497834 hasConcept C2777344606 @default.
- W2002497834 hasConcept C2780809830 @default.
- W2002497834 hasConcept C43617362 @default.
- W2002497834 hasConcept C543025807 @default.
- W2002497834 hasConcept C55493867 @default.
- W2002497834 hasConcept C75520062 @default.
- W2002497834 hasConceptScore W2002497834C181199279 @default.
- W2002497834 hasConceptScore W2002497834C185592680 @default.
- W2002497834 hasConceptScore W2002497834C2776317432 @default.
- W2002497834 hasConceptScore W2002497834C2777344606 @default.
- W2002497834 hasConceptScore W2002497834C2780809830 @default.
- W2002497834 hasConceptScore W2002497834C43617362 @default.
- W2002497834 hasConceptScore W2002497834C543025807 @default.
- W2002497834 hasConceptScore W2002497834C55493867 @default.
- W2002497834 hasConceptScore W2002497834C75520062 @default.
- W2002497834 hasIssue "2" @default.
- W2002497834 hasLocation W20024978341 @default.
- W2002497834 hasLocation W20024978342 @default.
- W2002497834 hasOpenAccess W2002497834 @default.
- W2002497834 hasPrimaryLocation W20024978341 @default.
- W2002497834 hasRelatedWork W1510038873 @default.
- W2002497834 hasRelatedWork W1983600898 @default.
- W2002497834 hasRelatedWork W1993549296 @default.
- W2002497834 hasRelatedWork W2032079981 @default.
- W2002497834 hasRelatedWork W2099766521 @default.
- W2002497834 hasRelatedWork W2111297576 @default.
- W2002497834 hasRelatedWork W2123115786 @default.
- W2002497834 hasRelatedWork W2143734654 @default.
- W2002497834 hasRelatedWork W2161994171 @default.
- W2002497834 hasRelatedWork W4224557617 @default.
- W2002497834 hasVolume "1081" @default.
- W2002497834 isParatext "false" @default.
- W2002497834 isRetracted "false" @default.
- W2002497834 magId "2002497834" @default.
- W2002497834 workType "article" @default.