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• W2002581213 abstract "Abstract Chick embryo fibroblasts (CEF) were infected with the Prague strain (subgroup C) of RSV (PrC-RSV) under conditions of rapid transformation and the synthesis of RNA and DNA capable of hybridizing with single-stranded DNA transcripts of 70 S PrC-RSV RNA (PrC-RSV DNA) was measured by hybridization kinetics analysis. PrC-RSV DNA was synthesized in the presence of high concentrations of actinomycin D and was freed from double-stranded DNA by chromatography; it protected over 90% of 70 S PrC-RSV RNA from digestion by ribonuclease at DNA:RNA ratios greater than 9. Virus-specific RNA sequences were measured with cellular RNA in vast excess, using the hybridization kinetics of the PrC-RSV DNA with 70 S PrC-RSV RNA as the reference. Virus-specific DNA sequences were measured with cellular DNA in vast excess, using the kinetics of self-annealing of the unique sequences of cellular DNA as the reference. Uninfected gs − CEF contained on the average one viral genome copy of DNA per cell and no detectable virus-specific RNA. DNA capable of hybridizing with the probe began to be synthesized within 3 hr after infection, and by 24 hr 2 viral genome copies had been synthesized. No additional virus-specific DNA was synthesized thereafter. During the first 6 hr after infection, CEF contained about 40 viral genome equivalents of RNA per cell, about 85% of which were in the cytoplasm. This represented most probably RNA in inoculum virus. Between 6 and 24 hr the number of viral genome equivalents per cell increased to 100. This newly synthesized RNA was equally distributed between the nucleus and polyribosomes. Between 24 and 96 hr the amount of virus-specific RNA in the nucleus and in polyribosomes increased by just under 2-fold, but in the remainder of the cytoplasm it rose by over 8-fold. By 96 hr cells contained about 340 genome equivalents of RNA per cell, which was about one-half of the number present in cells 14 days after infection. The size of the virus-specific RNA present in polyribosomes was predominantly 35 S, but some 10–30 S material was also present." @default.
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• W2002581213 title "Early synthesis of virus-specific RNA and DNA in cells rapidly transformed with Rous sarcoma virus" @default.
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• W2002581213 doi "https://doi.org/10.1016/0042-6822(73)90056-1" @default.
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