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- W2002585892 abstract "Agarose and polyacrylamide gel electrophoresis systems for the molecular mass-dependent separation of hyaluronan (HA) in the size range of approximately 5-500 kDa were investigated. For agarose-based systems, the suitability of different agarose types, agarose concentrations, and buffer systems was determined. Using chemoenzymatically synthesized HA standards of low polydispersity, the molecular mass range was determined for each gel composition over which the relationship between HA mobility and logarithm of the molecular mass was linear. Excellent linear calibration was obtained for HA molecular mass as low as approximately 9 kDa in agarose gels. For higher resolution separation, and for extension to molecular masses as low as approximately 5 kDa, gradient polyacrylamide gels were superior. Densitometric scanning of stained gels allowed analysis of the range of molecular masses present in a sample as well as calculation of weight-average and number-average values. The methods were validated for polydisperse HA samples with viscosity-average molecular masses of 112, 59, 37, and 22 kDa at sample loads of 0.5 μg (for polyacrylamide) to 2.5 μg (for agarose). Use of the methods for electrophoretic mobility shift assays was demonstrated for binding of the HA-binding region of aggrecan (recombinant human aggrecan G1-IGD-G2 domains) to a 150-kDa HA standard." @default.
- W2002585892 created "2016-06-24" @default.
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- W2002585892 date "2011-10-01" @default.
- W2002585892 modified "2023-10-18" @default.
- W2002585892 title "Agarose and polyacrylamide gel electrophoresis methods for molecular mass analysis of 5- to 500-kDa hyaluronan" @default.
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- W2002585892 cites W1964279075 @default.
- W2002585892 cites W1964485526 @default.
- W2002585892 cites W1966422114 @default.
- W2002585892 cites W1967227961 @default.
- W2002585892 cites W1970976508 @default.
- W2002585892 cites W1977868787 @default.
- W2002585892 cites W1980758295 @default.
- W2002585892 cites W1985007912 @default.
- W2002585892 cites W1986312103 @default.
- W2002585892 cites W1986909751 @default.
- W2002585892 cites W1998905953 @default.
- W2002585892 cites W2000489807 @default.
- W2002585892 cites W2002552794 @default.
- W2002585892 cites W2003380614 @default.
- W2002585892 cites W2006068421 @default.
- W2002585892 cites W2007028946 @default.
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- W2002585892 cites W2013379505 @default.
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- W2002585892 cites W2019487637 @default.
- W2002585892 cites W2021561467 @default.
- W2002585892 cites W2027260131 @default.
- W2002585892 cites W2027986481 @default.
- W2002585892 cites W2030295847 @default.
- W2002585892 cites W2033177835 @default.
- W2002585892 cites W2033386555 @default.
- W2002585892 cites W2043988634 @default.
- W2002585892 cites W2044475547 @default.
- W2002585892 cites W2053405152 @default.
- W2002585892 cites W2053802748 @default.
- W2002585892 cites W2057276491 @default.
- W2002585892 cites W2061626698 @default.
- W2002585892 cites W2062729550 @default.
- W2002585892 cites W2064611836 @default.
- W2002585892 cites W2069090516 @default.
- W2002585892 cites W2070442334 @default.
- W2002585892 cites W2071006316 @default.
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- W2002585892 cites W2076220323 @default.
- W2002585892 cites W2077425329 @default.
- W2002585892 cites W2079784375 @default.
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- W2002585892 cites W2082000832 @default.
- W2002585892 cites W2082815320 @default.
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- W2002585892 cites W2089753996 @default.
- W2002585892 cites W2100881010 @default.
- W2002585892 cites W2107166923 @default.
- W2002585892 cites W2107214690 @default.
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- W2002585892 cites W2123429740 @default.
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- W2002585892 cites W2134819882 @default.
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- W2002585892 doi "https://doi.org/10.1016/j.ab.2011.05.026" @default.
- W2002585892 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3207642" @default.
- W2002585892 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21684248" @default.
- W2002585892 hasPublicationYear "2011" @default.
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