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- W2002654773 abstract "Mitochondrial (mt) tRNA(Met) has the unusual modified nucleotide 5-formylcytidine (f(5)C) in the first position of the anticodon. This tRNA must translate both AUG and AUA as methionine. By constructing an in vitro translation system from bovine liver mitochondria, we examined the decoding properties of the native mt tRNA(Met) carrying f(5)C in the anticodon compared to a transcript that lacks the modification. The native mt Met-tRNA could recognize both AUA and AUG codons as Met, but the corresponding synthetic tRNA(Met) lacking f(5)C (anticodon CAU), recognized only the AUG codon in both the codon-dependent ribosomal binding and in vitro translation assays. Furthermore, the Escherichia coli elongator tRNA(Met)(m) with the anticodon ac(4)CAU (ac(4)C = 4-acetylcytidine) and the bovine cytoplasmic initiator tRNA(Met) (anticodon CAU) translated only the AUG codon for Met on mt ribosome. The codon recognition patterns of these tRNAs were the same on E. coli ribosomes. These results demonstrate that the f(5)C modification in mt tRNA(Met) plays a crucial role in decoding the nonuniversal AUA codon as Met, and that the genetic code variation is compensated by a change in the tRNA anticodon, not by a change in the ribosome. Base pairing models of f(5)C-G and f(5)C-A based on the chemical properties of f(5)C are presented." @default.
- W2002654773 created "2016-06-24" @default.
- W2002654773 creator A5044960150 @default.
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- W2002654773 creator A5075543753 @default.
- W2002654773 creator A5083952135 @default.
- W2002654773 date "2009-01-16" @default.
- W2002654773 modified "2023-10-13" @default.
- W2002654773 title "Unconventional decoding of the AUA codon as methionine by mitochondrial tRNA Met with the anticodon f 5 CAU as revealed with a mitochondrial in vitro translation system" @default.
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- W2002654773 cites W1516862523 @default.
- W2002654773 cites W1527548437 @default.
- W2002654773 cites W1554617000 @default.
- W2002654773 cites W1566509230 @default.
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- W2002654773 cites W1978925476 @default.
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- W2002654773 doi "https://doi.org/10.1093/nar/gkp001" @default.
- W2002654773 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2655697" @default.
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- W2002654773 hasPublicationYear "2009" @default.
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