FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2002685280> ?p ?o ?g. }

• W2002685280 endingPage "396" @default.
• W2002685280 startingPage "388" @default.
• W2002685280 abstract "B104, an established rat neuroblastoma cell line exhibiting specific neuronal qualities, was chosen as a model to study insulin-like growth factor (IGF) binding and action in the central nervous system. Specific binding of [125I]IGF-II to B104 membranes averaged 12.2 +/- 4.0% (mean +/- SD)/100 micrograms/ml protein compared with [125I]IGF-I binding of 10.1 +/- 2.9%. In competitive binding studies employing [125I]IGF-II as the radioligand, high affinity for IGF-II was demonstrated (50% displacement at 2.7 ng/ml), with none for IGF-I or insulin. Upon cross-linking [125I]IGF-I to membranes under reducing conditions, two prominent bands were observed, migrating with apparent mol wt (Mr) of 135,000 and 280,000. Both bands were inhibited by IGFs and insulin, but not by R-II-PABI, a polyclonal antibody to the type 2 receptor. These bands presumably represent the alpha-subunit and an incompletely reduced alpha-alpha-dimer of the type 1 IGF receptor. When cross-linking [125I]IGF-II to membranes under reducing conditions, the primary labeled bands migrated with apparent Mr of 260,000 and 280,000. These bands were inhibited by IGF-II and R-II-PABI, but not by insulin, and probably represent the monomeric type 2 receptor. In addition, we observed a minor band at apparent Mr 35,000, which was inhibited by IGF but not by insulin. By a modified cross-linking technique, we confirmed the existence of a small IGF-binding protein in the serum-free conditioned medium of B104 cultures, migrating as two bands with apparent Mr of 33,000-39,000. These proteins demonstrated high affinity for IGF-I and IGF-II, but none for insulin. In summary, this study demonstrates the presence in B104 rat neuroblastoma cells of 1) abundant classical type 1 and type 2 IGF receptors, and 2) a secreted and membrane-associated small IGF-binding protein." @default.
• W2002685280 created "2016-06-24" @default.
• W2002685280 creator A5001402556 @default.
• W2002685280 creator A5045874641 @default.
• W2002685280 creator A5051439803 @default.
• W2002685280 creator A5073833030 @default.
• W2002685280 date "1989-01-01" @default.
• W2002685280 modified "2023-09-27" @default.
• W2002685280 title "Insulin-Like Growth Factor Receptors and Binding Protein in Rat Neuroblastoma Cells*" @default.
• W2002685280 cites W1501458107 @default.
• W2002685280 cites W1543624854 @default.
• W2002685280 cites W1590558744 @default.
• W2002685280 cites W1838892598 @default.
• W2002685280 cites W1893651774 @default.
• W2002685280 cites W1972572631 @default.
• W2002685280 cites W1994734038 @default.
• W2002685280 cites W1995535256 @default.
• W2002685280 cites W1998225973 @default.
• W2002685280 cites W2010178983 @default.
• W2002685280 cites W2013551015 @default.
• W2002685280 cites W2018002682 @default.
• W2002685280 cites W2020644093 @default.
• W2002685280 cites W2021008086 @default.
• W2002685280 cites W2024683111 @default.
• W2002685280 cites W2027152719 @default.
• W2002685280 cites W2032558447 @default.
• W2002685280 cites W2032737002 @default.
• W2002685280 cites W2036492185 @default.
• W2002685280 cites W2038792569 @default.
• W2002685280 cites W2040648948 @default.
• W2002685280 cites W2041108328 @default.
• W2002685280 cites W2042211739 @default.
• W2002685280 cites W2044998331 @default.
• W2002685280 cites W2048727629 @default.
• W2002685280 cites W2059037233 @default.
• W2002685280 cites W2061762581 @default.
• W2002685280 cites W2063994101 @default.
• W2002685280 cites W2070456428 @default.
• W2002685280 cites W2078680796 @default.
• W2002685280 cites W2084007285 @default.
• W2002685280 cites W2086017926 @default.
• W2002685280 cites W2089468998 @default.
• W2002685280 cites W2090059609 @default.
• W2002685280 cites W2097257515 @default.
• W2002685280 cites W2159357044 @default.
• W2002685280 cites W2164051143 @default.
• W2002685280 cites W2164929274 @default.
• W2002685280 cites W2461875665 @default.
• W2002685280 doi "https://doi.org/10.1210/endo-124-1-388" @default.
• W2002685280 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2462492" @default.
• W2002685280 hasPublicationYear "1989" @default.
• W2002685280 type Work @default.
• W2002685280 sameAs 2002685280 @default.
• W2002685280 citedByCount "34" @default.
• W2002685280 countsByYear W20026852802019 @default.
• W2002685280 countsByYear W20026852802020 @default.
• W2002685280 crossrefType "journal-article" @default.
• W2002685280 hasAuthorship W2002685280A5001402556 @default.
• W2002685280 hasAuthorship W2002685280A5045874641 @default.
• W2002685280 hasAuthorship W2002685280A5051439803 @default.
• W2002685280 hasAuthorship W2002685280A5073833030 @default.
• W2002685280 hasConcept C104317684 @default.
• W2002685280 hasConcept C107824862 @default.
• W2002685280 hasConcept C126322002 @default.
• W2002685280 hasConcept C134018914 @default.
• W2002685280 hasConcept C159654299 @default.
• W2002685280 hasConcept C170493617 @default.
• W2002685280 hasConcept C185592680 @default.
• W2002685280 hasConcept C203014093 @default.
• W2002685280 hasConcept C2775960820 @default.
• W2002685280 hasConcept C2776715637 @default.
• W2002685280 hasConcept C2779069268 @default.
• W2002685280 hasConcept C2779306644 @default.
• W2002685280 hasConcept C2780689927 @default.
• W2002685280 hasConcept C41625074 @default.
• W2002685280 hasConcept C54355233 @default.
• W2002685280 hasConcept C553089730 @default.
• W2002685280 hasConcept C55493867 @default.
• W2002685280 hasConcept C67847695 @default.
• W2002685280 hasConcept C71924100 @default.
• W2002685280 hasConcept C80862487 @default.
• W2002685280 hasConcept C81885089 @default.
• W2002685280 hasConcept C86803240 @default.
• W2002685280 hasConcept C956191 @default.
• W2002685280 hasConceptScore W2002685280C104317684 @default.
• W2002685280 hasConceptScore W2002685280C107824862 @default.
• W2002685280 hasConceptScore W2002685280C126322002 @default.
• W2002685280 hasConceptScore W2002685280C134018914 @default.
• W2002685280 hasConceptScore W2002685280C159654299 @default.
• W2002685280 hasConceptScore W2002685280C170493617 @default.
• W2002685280 hasConceptScore W2002685280C185592680 @default.
• W2002685280 hasConceptScore W2002685280C203014093 @default.
• W2002685280 hasConceptScore W2002685280C2775960820 @default.
• W2002685280 hasConceptScore W2002685280C2776715637 @default.
• W2002685280 hasConceptScore W2002685280C2779069268 @default.
• W2002685280 hasConceptScore W2002685280C2779306644 @default.
• W2002685280 hasConceptScore W2002685280C2780689927 @default.
• W2002685280 hasConceptScore W2002685280C41625074 @default.

• next