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• W2002705908 abstract "The process of stack coalescence, an important mechanism of Golgi recovery from mitosis, was examined using novel experimental paradigms. In living cells with disrupted (by nocodazole) microtubules, galactosyl transferase-GFP-Iabelled Golgi fragments constantly appeared, grew, sometimes moved with a speed of 1–2 µm/min, coalesced or graduaUy diminished and disappeared. The rate of Golgi fragment turnover and coalescence was highly balanced to maintain a constant number of Golgi units per cell. Moreover some Golgi islands appear and some received new GalTase-GFP after photobleaching of cell cytoplasm. Short tubules extending from the rims of scattered Golgi fragments frequently formed bridges between ministacks, inducing their coalescence. The frequency of coalescence could also be inhibited by disruption of actin micrordaments. After the Golgi redistribution into endoplasmic reticulum induced by brefeldin A, either the growth of small Golgi fragments or tbeir coalescence leads to compartmentalized stack formation without the participation of microtubules. These results demonstrate that this coalescence between isolated Golgi stacks is microtubule-independent and could thus be mediated by membranous tubules." @default.
• W2002705908 created "2016-06-24" @default.
• W2002705908 creator A5053837862 @default.
• W2002705908 creator A5070435916 @default.
• W2002705908 creator A5090295114 @default.
• W2002705908 date "1999-03-01" @default.
• W2002705908 modified "2023-10-16" @default.
• W2002705908 title "Coalescence of Golgi fragments in microtubule-deprived living cells" @default.
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• W2002705908 doi "https://doi.org/10.1016/s0171-9335(99)80096-x" @default.
• W2002705908 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10219567" @default.
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