FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2002767537> ?p ?o ?g. }

• W2002767537 endingPage "161" @default.
• W2002767537 startingPage "153" @default.
• W2002767537 abstract "The Mycobacterium avium subspecies (MAs) include the closely related MAs avium and MAs paratuberculosis. This study was conducted to evaluate the performance of a PCR panel assay as a diagnostic tool to detect and differentiate MAs avium and MAs paratuberculosis infection. Specific oligonucleotides primers derived from the 16 S rRNA (MAs) sequence, insertion elements IS 901 (MAs avium), IS 1245 Mycobacterium avium complex (MAC), IS 900 (MAs paratuberculosis), and the hspX (MAs paratuberculosis) gene sequences were synthesized and used in preassembled PCR reaction mixtures. These five primer sets made up the PCR panel assay. To determine the accuracy of the PCR panel assay for MAs avium and MAs paratuberculosis strain detection and differentiation, lysates of mycobacterial DNA from 120 (n=120) strains were tested with the PCR panel assay by one laboratory (#1). The PCR panel assay specifically detected and differentiated 91/91 (100%) of MAs avium and MAs paratuberculosis strains tested in this study. The PCR panel assay also specifically differentiated all MAs avium and MAs paratuberculosis strains from all but one (M. intracellulare, serovar 23) of the other mycobacterial strains tested. To confirm the accuracy and evaluate the reproducibility of the PCR panel assay, samples were numbered and given to a different laboratory (#2) as 'unknowns' for identification by the PCR panel assay. In this study, the overall accuracy for strain identification using the PCR panel assay was 99.2% (119/120). The reproducibility of the PCR panel assay when comparing data from laboratory #1 with laboratory #2 was found to be 100% (120/120). These results indicate that this 'easy-to-use', rapid PCR method can accurately and reliably detect and differentiate closely related MAs avium and MAs paratuberculosis from each other and from other mycobacterial species. The PCR panel assay can also differentiate mixed cultures of MAs. The simplicity of this PCR method could be beneficial to laboratories that test for members of MA." @default.
• W2002767537 created "2016-06-24" @default.
• W2002767537 creator A5007150347 @default.
• W2002767537 creator A5058110423 @default.
• W2002767537 creator A5059460889 @default.
• W2002767537 creator A5065603528 @default.
• W2002767537 creator A5089421422 @default.
• W2002767537 date "2000-06-01" @default.
• W2002767537 modified "2023-10-14" @default.
• W2002767537 title "Evaluation of the accuracy and reproducibility of a practical PCR panel assay for rapid detection and differentiation ofMycobacterium avium subspecies" @default.
• W2002767537 cites W1602908224 @default.
• W2002767537 cites W1632672891 @default.
• W2002767537 cites W1649147452 @default.
• W2002767537 cites W1649285159 @default.
• W2002767537 cites W1692954654 @default.
• W2002767537 cites W1830500408 @default.
• W2002767537 cites W1917102629 @default.
• W2002767537 cites W1928917901 @default.
• W2002767537 cites W2009891323 @default.
• W2002767537 cites W2012615444 @default.
• W2002767537 cites W2016613682 @default.
• W2002767537 cites W2022400760 @default.
• W2002767537 cites W2026271313 @default.
• W2002767537 cites W2028199279 @default.
• W2002767537 cites W2034389965 @default.
• W2002767537 cites W2056863096 @default.
• W2002767537 cites W2062093964 @default.
• W2002767537 cites W2098981750 @default.
• W2002767537 cites W2107177029 @default.
• W2002767537 cites W2112991848 @default.
• W2002767537 cites W2121382364 @default.
• W2002767537 cites W2126412048 @default.
• W2002767537 cites W2133573744 @default.
• W2002767537 cites W2140534296 @default.
• W2002767537 cites W2145737007 @default.
• W2002767537 cites W2153663358 @default.
• W2002767537 cites W2155186392 @default.
• W2002767537 cites W2164951681 @default.
• W2002767537 cites W2249188126 @default.
• W2002767537 cites W4211007841 @default.
• W2002767537 doi "https://doi.org/10.1006/mcpr.2000.0299" @default.
• W2002767537 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10860713" @default.
• W2002767537 hasPublicationYear "2000" @default.
• W2002767537 type Work @default.
• W2002767537 sameAs 2002767537 @default.
• W2002767537 citedByCount "35" @default.
• W2002767537 countsByYear W20027675372012 @default.
• W2002767537 countsByYear W20027675372013 @default.
• W2002767537 countsByYear W20027675372014 @default.
• W2002767537 countsByYear W20027675372017 @default.
• W2002767537 countsByYear W20027675372018 @default.
• W2002767537 countsByYear W20027675372023 @default.
• W2002767537 crossrefType "journal-article" @default.
• W2002767537 hasAuthorship W2002767537A5007150347 @default.
• W2002767537 hasAuthorship W2002767537A5058110423 @default.
• W2002767537 hasAuthorship W2002767537A5059460889 @default.
• W2002767537 hasAuthorship W2002767537A5065603528 @default.
• W2002767537 hasAuthorship W2002767537A5089421422 @default.
• W2002767537 hasConcept C10389963 @default.
• W2002767537 hasConcept C104317684 @default.
• W2002767537 hasConcept C153911025 @default.
• W2002767537 hasConcept C159047783 @default.
• W2002767537 hasConcept C178790620 @default.
• W2002767537 hasConcept C185592680 @default.
• W2002767537 hasConcept C2777563447 @default.
• W2002767537 hasConcept C2777849792 @default.
• W2002767537 hasConcept C2780015948 @default.
• W2002767537 hasConcept C2780374374 @default.
• W2002767537 hasConcept C2909019056 @default.
• W2002767537 hasConcept C43617362 @default.
• W2002767537 hasConcept C49105822 @default.
• W2002767537 hasConcept C523546767 @default.
• W2002767537 hasConcept C54355233 @default.
• W2002767537 hasConcept C86803240 @default.
• W2002767537 hasConcept C89423630 @default.
• W2002767537 hasConcept C9893847 @default.
• W2002767537 hasConceptScore W2002767537C10389963 @default.
• W2002767537 hasConceptScore W2002767537C104317684 @default.
• W2002767537 hasConceptScore W2002767537C153911025 @default.
• W2002767537 hasConceptScore W2002767537C159047783 @default.
• W2002767537 hasConceptScore W2002767537C178790620 @default.
• W2002767537 hasConceptScore W2002767537C185592680 @default.
• W2002767537 hasConceptScore W2002767537C2777563447 @default.
• W2002767537 hasConceptScore W2002767537C2777849792 @default.
• W2002767537 hasConceptScore W2002767537C2780015948 @default.
• W2002767537 hasConceptScore W2002767537C2780374374 @default.
• W2002767537 hasConceptScore W2002767537C2909019056 @default.
• W2002767537 hasConceptScore W2002767537C43617362 @default.
• W2002767537 hasConceptScore W2002767537C49105822 @default.
• W2002767537 hasConceptScore W2002767537C523546767 @default.
• W2002767537 hasConceptScore W2002767537C54355233 @default.
• W2002767537 hasConceptScore W2002767537C86803240 @default.
• W2002767537 hasConceptScore W2002767537C89423630 @default.
• W2002767537 hasConceptScore W2002767537C9893847 @default.
• W2002767537 hasIssue "3" @default.
• W2002767537 hasLocation W20027675371 @default.
• W2002767537 hasLocation W20027675372 @default.
• W2002767537 hasOpenAccess W2002767537 @default.

• next