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- W2002793841 abstract "Abstract Chemiluminescence assays are used widely for the detection of nitric oxide (NO)-derived species in biological fluids and tissues. Here, we demonstrate that these assays can be interfaced with mass-sensitive detectors for parallel determination of isotopic abundance. Results obtained with tri-iodide and ascorbic acid-based reductive assays indicate that mass spectrometric detection enables NO isotope-tracing experiments to be carried out to a limit of detectability of a few picomoles, a sensitivity similar to that of standard gas phase chemiluminescence methods. The advantage afforded by mass spectrometric detection is demonstrated using the murine macrophage cell line J774, which is shown here to reduce 15 NO 3 - to 15 NO 2 - under anoxic conditions. The particular combination of an analytical and cellular system described here may hold promise for future characterization of the enzymatic pathways contributing to mammalian nitrate reductase activity, without background interference from 14 NO 2 - derived from other sources." @default.
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- W2002793841 date "2008-11-29" @default.
- W2002793841 modified "2023-09-26" @default.
- W2002793841 title "Isotope tracing enhancement of chemiluminescence assays for nitric oxide research" @default.
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- W2002793841 doi "https://doi.org/10.1515/bc.2009.017" @default.
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