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- W2002805166 abstract "The ubiquitous nature of the Alu sequence throughout the human genome forms the basis of an assay we present here for analyzing the human chromosome content of human × rodent somatic cell hybrids. A human-specific Alu primer was used both to amplify sequences and to 32P label the products in a polymerase chain reaction (PCR) technique. Unlabeled inter-Alu PCR products from two series of human × rodent hybrids were used to prepare dot blots which were probed with labeled inter-Alu products prepared from between 103 and 104 hybrid cells. In the first series we demonstrate that a labeled inter-Alu probe from the hybrid DL18ts, containing a single chromosome 18, on a dot blot hybridized only with those inter-Alu products containing chromosome 18. Similar specificity for human chromosome 5 was shown when a Southern blot of the PCR products was hybridized with a probe made from the hybrid HHW 213, which contains only chromosome 5p. Using a dot blot from a second series of control hybrids, 15 of which contained single human chromosomes, hybridization of a labeled probe from the hybrid 18X4-1 was shown to react specifically with the controls that expressed chromosome 18. Application of the technique reported here allows simple and rapid characterization of the human chromosome content in human × rodent hybrids." @default.
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- W2002805166 date "1991-05-01" @default.
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- W2002805166 title "The human chromosome content in human × rodent somatic cell hybrids analyzed by a screening technique using Alu PCR" @default.
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- W2002805166 doi "https://doi.org/10.1016/0888-7543(91)90499-5" @default.
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