Matches in SemOpenAlex for { <https://semopenalex.org/work/W2002807739> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2002807739 endingPage "106" @default.
- W2002807739 startingPage "99" @default.
- W2002807739 abstract "The pathology of Parkinson's disease involves oxidative damage to dopaminergic neurons of the substantia nigra. Oxidation of the dopamine (DA) neurotransmitter itself may contribute to the generation of a reactive oxygen species (ROS) and subsequent neurodegeneration. Glia cells can either exacerbate injury or exert protective properties on local neurons in the brain. We investigate glial antioxidant enzyme systems relative to ROS generated during cytokine activation, monoamine oxidase (MAO) activity and autoxidation of DA in glioma cells. Rat C6 glioma cells stimulated with lipopolysaccharide Escherichia coli 0111:B4 and interferon gamma (LPS/IFN-g) produced high levels of nitric oxide (241 nmol mg−1 protein 24 h−1) but not superoxide (O−2) or hydrogen peroxide (H2O2). Basal C6 cells exhibited a rapid and robust capacity to remove exogenous H2O2 within minutes. Preincubation with sodium azide but not buthionine-[S, R]-sulfoximine attenuated this response, indicating catalase as the primary enzyme responsible for this effect. The glioma catalase reaction rate was slightly attenuated by exposure to LPS/IFN-g for 24 h. However, the reduction in catalase activity was not due to nitric oxide, because both the supernatant and sodium nitroprusside had no effect on isolated catalase enzyme activity. Hydrogen peroxide was produced only through substrate-driven MAO activity in prepared lysate. However, the quantity of H2O2 produced per unit time (0.46 nmol mg−1 protein min−1) was negligible compared with the enormous capacity for its removal by catalase (213.9 nmol mg−1 protein min−1) (≥462× greater). Similarly, H2O2 generated by DA autoxidation per unit time (0.28 nmol mg−1 protein equiv. min−1), was rapidly dissolved by glioma cells at high capacity (≥750× greater). In conclusion, C6 cells produce nitric oxide under cytokine/endotoxin-stimulated conditions. Moreover, C6 cells exhibit a dynamic H2O2 scavenging capacity, with ample facility to dispose of the peroxide generated by both MAO activity and spontaneous DA autoxidation. Copyright © 2004 John Wiley & Sons, Ltd." @default.
- W2002807739 created "2016-06-24" @default.
- W2002807739 creator A5035760747 @default.
- W2002807739 creator A5056430801 @default.
- W2002807739 date "2004-03-01" @default.
- W2002807739 modified "2023-09-23" @default.
- W2002807739 title "Glioma cell antioxidant capacity relative to reactive oxygen species produced by dopamine" @default.
- W2002807739 doi "https://doi.org/10.1002/jat.954" @default.
- W2002807739 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15052604" @default.
- W2002807739 hasPublicationYear "2004" @default.
- W2002807739 type Work @default.
- W2002807739 sameAs 2002807739 @default.
- W2002807739 citedByCount "56" @default.
- W2002807739 countsByYear W20028077392012 @default.
- W2002807739 countsByYear W20028077392014 @default.
- W2002807739 countsByYear W20028077392015 @default.
- W2002807739 countsByYear W20028077392017 @default.
- W2002807739 countsByYear W20028077392018 @default.
- W2002807739 countsByYear W20028077392019 @default.
- W2002807739 countsByYear W20028077392020 @default.
- W2002807739 countsByYear W20028077392021 @default.
- W2002807739 countsByYear W20028077392022 @default.
- W2002807739 crossrefType "journal-article" @default.
- W2002807739 hasAuthorship W2002807739A5035760747 @default.
- W2002807739 hasAuthorship W2002807739A5056430801 @default.
- W2002807739 hasConcept C178790620 @default.
- W2002807739 hasConcept C185592680 @default.
- W2002807739 hasConcept C2776151105 @default.
- W2002807739 hasConcept C2777209548 @default.
- W2002807739 hasConcept C2778004101 @default.
- W2002807739 hasConcept C2778979269 @default.
- W2002807739 hasConcept C2779610285 @default.
- W2002807739 hasConcept C2779719074 @default.
- W2002807739 hasConcept C48349386 @default.
- W2002807739 hasConcept C519581460 @default.
- W2002807739 hasConcept C533411734 @default.
- W2002807739 hasConcept C55493867 @default.
- W2002807739 hasConceptScore W2002807739C178790620 @default.
- W2002807739 hasConceptScore W2002807739C185592680 @default.
- W2002807739 hasConceptScore W2002807739C2776151105 @default.
- W2002807739 hasConceptScore W2002807739C2777209548 @default.
- W2002807739 hasConceptScore W2002807739C2778004101 @default.
- W2002807739 hasConceptScore W2002807739C2778979269 @default.
- W2002807739 hasConceptScore W2002807739C2779610285 @default.
- W2002807739 hasConceptScore W2002807739C2779719074 @default.
- W2002807739 hasConceptScore W2002807739C48349386 @default.
- W2002807739 hasConceptScore W2002807739C519581460 @default.
- W2002807739 hasConceptScore W2002807739C533411734 @default.
- W2002807739 hasConceptScore W2002807739C55493867 @default.
- W2002807739 hasIssue "2" @default.
- W2002807739 hasLocation W20028077391 @default.
- W2002807739 hasLocation W20028077392 @default.
- W2002807739 hasOpenAccess W2002807739 @default.
- W2002807739 hasPrimaryLocation W20028077391 @default.
- W2002807739 hasRelatedWork W1443871574 @default.
- W2002807739 hasRelatedWork W1586446098 @default.
- W2002807739 hasRelatedWork W2128951234 @default.
- W2002807739 hasRelatedWork W2312976037 @default.
- W2002807739 hasRelatedWork W2334246894 @default.
- W2002807739 hasRelatedWork W2350255099 @default.
- W2002807739 hasRelatedWork W2395950097 @default.
- W2002807739 hasRelatedWork W2596329378 @default.
- W2002807739 hasRelatedWork W2737873067 @default.
- W2002807739 hasRelatedWork W3214998341 @default.
- W2002807739 hasVolume "24" @default.
- W2002807739 isParatext "false" @default.
- W2002807739 isRetracted "false" @default.
- W2002807739 magId "2002807739" @default.
- W2002807739 workType "article" @default.