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• W2002809077 abstract "To evaluate the peptidoglycan-associated lipoprotein (PAL) antigen of Legionella pneumophila as a vaccine candidate, mice were immunized intramuscularly with pcDNA3-PAL and intraperitoneally with recombinant PAL (t-rPAL), which were compared for their ability to induce PAL-specific immune responses. The t-rPAL protein induced PAL-specific IgG antibody production significantly more than did pcDNA3-PAL. The IgG2a and IgG1 production was predominant after pcDNA3-PAL and t-rPAL administration, respectively. In particular, pcDNA3-PAL induced much higher PAL-specific cytotoxic T-lymphocyte responses than did t-rPAL. Furthermore, in vivo, CD19+ B-cell populations were dramatically increased by t-rPAL vaccination, suggesting a B-cell immunomodulatory activity of the lipoprotein. The PAL antigen was also conserved among Legionella species, as determined by PCR and immunoblot analyses. These results support a potential use of the t-rPAL protein and in particular DNA vaccines against Legionella infections." @default.
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• W2002809077 date "2002-02-01" @default.
• W2002809077 modified "2023-09-26" @default.
• W2002809077 title "Comparison of Responses Elicited by Immunization with a<i>Legionella</i>Species Common Lipoprotein Delivered as Naked DNA or Recombinant Protein" @default.
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• W2002809077 doi "https://doi.org/10.1089/104454902753604970" @default.
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