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- W2002809303 abstract "Bromopyruvate is an alkylating agent of pigeon liver malic enzyme (malate dehydrogenase (decarboxylating), EC 1.1.1.40). It combines first with the enzyme to give an enzyme-bromopyruvate complex, then reacts with a proximal -SH group, resulting in the formation of a pyruvate derivative. Bromopyruvate is also a substrate for the reductase partial reaction, and a non-competitive inhibitor of L-malate in the overall oxidative decarboxylase reaction catalyzed by this enzyme. Modification of the -SH group by this compound is accompanied by concomitant loss of both oxidative decarboxylase activity and reductase activity on bromopyruvate. Inactivation of the overall activity is partially prevented by NADP+ or NADPH, singly or in combination with L-malate." @default.
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- W2002809303 date "1973-12-01" @default.
- W2002809303 modified "2023-09-27" @default.
- W2002809303 title "The substrate analog bromopyruvate as a substrate, an inhibitor and an alkylating agent of malic enzyme of pigeon liver" @default.
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- W2002809303 doi "https://doi.org/10.1016/0006-291x(73)91183-2" @default.
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