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- W2002846412 abstract "Extracellular oxygen concentrations influence cell metabolism and tissue function. Fluorescence Lifetime ImagingMicroscopy (FLIM) offers a non-invasive method for quantifying local oxygen concentrations. However, existingmethods show limited spatial resolution and/or require custom made systems. This study describes a new optimisedapproach for quantitative extracellular oxygen detection, providing an off-the-shelf system with high spatial resolutionand an improved lifetime determination over previous techniques, while avoiding systematic photon pile-up.Fluorescence lifetime detection of an oxygen sensitive fluorescent dye, tris(2,2'-bipyridyl)ruthenium(II) chloridehexahydrate [Ru(bipy)3]2+, was measured using a Becker&Hickl time-correlated single photon counting (TCSPC) cardwith excitation provided by a multi-photon laser. This technique was able to identify a subpopulation of isolatedchondrocyte cells, seeded in three-dimensional agarose gel, displaying a significant spatial oxygen gradient. Thus thistechnique provides a powerful tool for quantifying spatial oxygen gradients within three-dimensional cellular models." @default.
- W2002846412 created "2016-06-24" @default.
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- W2002846412 date "2010-02-11" @default.
- W2002846412 modified "2023-09-23" @default.
- W2002846412 title "Extracellular oxygen concentration mapping with a confocal multiphoton laser scanning microscope and TCSPC card" @default.
- W2002846412 doi "https://doi.org/10.1117/12.842281" @default.
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